ArticleScientific reports2017
Messenger RNAs localized to distal projections of human stem cell derived neurons.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
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- Reticulon-1 synthesis controls outgrowth and microtubule dynamics in injured cortical axons.Life science alliance · 2026Article
- Regulation of Subcellular Protein Synthesis for Restoring Neural Connectivity.International journal of molecular sciences · 2025Review
- Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration.Nature neuroscience · 2024Article
- Analysis of subcellular RNA fractions demonstrates significant genetic regulation of gene expression in human brain post-transcriptionally.Scientific reports · 2023Article
- Neurons and Astrocytes Elicit Brain Region Specific Transcriptional Responses to Prion Disease in the Murine CA1 and Thalamus.Frontiers in neuroscience · 2022Article
- Prediction of RNA subcellular localization: Learning from heterogeneous data sources.iScience · 2021Review
- Heat Shock Protein 70 as a Sex-Skewed Regulator of α-Synucleinopathy.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2021Article
- Facilitating mGluR4 activity reverses the long-term deleterious consequences of chronic morphine exposure in male mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021Article
- Functional Genomics of Axons and Synapses to Understand Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Review
- Axonal Transport and Local Translation of mRNA in Neurodegenerative Diseases.Frontiers in molecular neuroscience · 2021Review
- Neurodegeneration and axonal mRNA transportation.American journal of neurodegenerative disease · 2021Review
- CBeLife · 2020Article
- 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Dysregulation of protein synthesis and dendritic spine morphogenesis in ASD: studies in human pluripotent stem cells.Molecular autism · 2020Review
- Review
- Omics Approach to Axonal Dysfunction of Motor Neurons in Amyotrophic Lateral Sclerosis (ALS).Frontiers in neuroscience · 2020Review
- Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system.Scientific reports · 2019Article
- Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips.Journal of visualized experiments : JoVE · 2019Article
- Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The identification of mRNAs in distal projections of model organisms has led to the discovery of multiple proteins that are locally synthesized for functional roles such as axon guidance, injury signaling and regeneration. The extent to which local protein synthesis is conserved in human neurons is unknown. Here we used compartmentalized microfluidic chambers to characterize the transcriptome of distal projections of human embryonic stem cells differentiated using a protocol which enriched for glutamatergic neurons (hESC-neurons). Using gene expression analysis, we identified mRNAs proportionally enriched in these projections, representing a functionally unique local transcriptome as compared to the human neuronal transcriptome inclusive of somata. Further, we found that the most abundant mRNAs within these hESC-neuron projections were functionally similar to the axonal transcriptome of rat cortical neurons. We confirmed the presence of two well characterized axonal mRNAs in model organisms, β-actin and GAP43, within hESC-neuron projections using multiplexed single molecule RNA-FISH. Additionally, we report the novel finding that oxytocin mRNA localized to these human projections and confirmed its localization using RNA-FISH. This new evaluation of mRNA within human projections provides an important resource for studying local mRNA translation and has the potential to reveal both conserved and unique translation dependent mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.