ArticleParasites & vectors2017
The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 29 citations in OpenAlex.
- Genetic diversity of Toxoplasma gondii isolates from members of the families camelidae and equidae worldwide: a systematic review.Irish veterinary journal · 2026Review
- Effect of Parasitic Infections on Hematological Profile, Reproductive and Productive Performance in Equines.Animals : an open access journal from MDPI · 2025Review
- Isolation ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Early immune response toFrontiers in cellular and infection microbiology · 2024Article
- A Pork Industry in the Backyard: An Analysis ofMicroorganisms · 2023Article
- Comparison of a Commercial Enzyme-Linked Immunosorbent Assay (ELISA) with the Modified Agglutination Test (MAT) for the Detection of Antibodies againstAnimals : an open access journal from MDPI · 2022Article
- The Prevalence of Early Toxoplasmosis after Bone Marrow Transplantation Using PCR: A Study in Iranian Patients.Infectious disorders drug targets · 2022Article
- Article
- First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia.Scientific reports · 2021Article
- Parasitic diseases of equids in Iran (1931-2020): a literature review.Parasites & vectors · 2020Review
- In Vivo and In Vitro Virulence Analysis of Four Genetically DistinctMicroorganisms · 2020Article
- Article
- Prevalence and genetic characterization of Toxoplasma gondii in naturally infected backyard pigs intended for familial consumption in Romania.Parasites & vectors · 2019Article
- Evaluation ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Diversity ofFood and waterborne parasitology · 2019Review
- Review
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Authors and funding
12 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConsumption of undercooked or insufficiently cured meat is a major risk factor for human infection with Toxoplasma gondii. Although horsemeat is typically consumed rare or undercooked, information on the risk of T. gondii from infected horse meat to humans is scarce. Here, we present the results of a study to determine the presence of T. gondii infection in slaughter horses in Serbia, and to attempt to isolate viable parasites.
methodsThe study included horses from all regions of Serbia slaughtered at two abattoirs between June 2013 and June 2015. Blood sera were tested for the presence of specific IgG T. gondii antibodies by the modified agglutination test (MAT), and samples of trypsin-digested heart tissue were bioassayed in mice. Cyst-positive mouse brain homogenates were subjected to DNA extraction and T. gondii strains were genotyped using 15 microsatellite markers (MS).
resultsA total of 105 slaughter horses were sampled. At the 1:6 cut-off 48.6% of the examined horses were seropositive, with the highest titre being 1:400. Viable parasites were isolated from two grade type mares; both parasite isolates (RS-Eq39 and RS-Eq40) were T. gondii type III, and both displayed an increased lethality for mice with successive passages. These are the first cases of isolation of T. gondii from horses in Serbia. When compared with a worldwide collection of 61 type III and type III-like strains, isolate RS-Eq39 showed a combination of MS lengths similar to a strain isolated from a duck in Iran, and isolate RS-Eq40 was identical in all markers to three strains isolated from a goat from Gabon, a sheep from France and a pig from Portugal. Interestingly, the source horses were one seronegative and one weakly seropositive.
conclusionsThe isolation of viable T. gondii parasites from slaughter horses points to horsemeat as a potential source of human infection, but the fact that viable parasites were isolated from horses with only a serological trace of T. gondii infection presents further evidence that serology may not be adequate to assess the risk of toxoplasmosis from horsemeat consumption. Presence of T. gondii type III in Serbia sheds more light into the potential origin of this archetypal lineage in Europe.
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