Evidence map›Paper›PMID 28376803›Full record

ArticleBiomedical engineering online2017

Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling.

Guohua Bai, Ying Li, Henry K Chu, Kaiqun Wang, Qiulin Tan, Jijun Xiong, Dong Sun

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical engineering online, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
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  5. Lab on a chip · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Guohua BaiKey Laboratory of Instrumentation Science and Dynamic Measurement, Ministry of Education, North University of China, Room 418, Building No. 14, No. 3 Xueyuan Road, Taiyuan, 030051, Shanxi, China.
Ying LiDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, SAR of China.
Henry K ChuDepartment of Mechanical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR of China.
Kaiqun WangDepartment of Biomedical Engineering, College of Mechanics, Taiyuan University of Technology, No. 79, West Yingze Street, Taiyuan, 030024, Shanxi, China.
Qiulin TanKey Laboratory of Instrumentation Science and Dynamic Measurement, Ministry of Education, North University of China, Room 418, Building No. 14, No. 3 Xueyuan Road, Taiyuan, 030051, Shanxi, China.
Jijun XiongKey Laboratory of Instrumentation Science and Dynamic Measurement, Ministry of Education, North University of China, Room 418, Building No. 14, No. 3 Xueyuan Road, Taiyuan, 030051, Shanxi, China.
Dong SunDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, SAR of China. medsun@cityu.edu.hk.ORCID http://orcid.org/0000-0003-3945-4037
North University of China · CNCity University of Hong Kong · HKHong Kong Polytechnic University · HKTaiyuan University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCytoskeleton is a highly dynamic network that helps to maintain the rigidity of a cell, and the mechanical properties of a cell are closely related to many cellular functions. This paper presents a new method to probe and characterize cell mechanical properties through dielectrophoresis (DEP)-based cell stretching manipulation and actin cytoskeleton modeling.

methodsLeukemia NB4 cells were used as cell line, and changes in their biological properties were examined after chemotherapy treatment with doxorubicin (DOX). DEP-integrated microfluidic chip was utilized as a low-cost and efficient tool to study the deformability of cells. DEP forces used in cell stretching were first evaluated through computer simulation, and the results were compared with modeling equations and with the results of optical stretching (OT) experiments. Structural parameters were then extracted by fitting the experimental data into the actin cytoskeleton model, and the underlying mechanical properties of the cells were subsequently characterized.

resultsThe DEP forces generated under different voltage inputs were calculated and the results from different approaches demonstrate good approximations to the force estimation. Both DEP and OT stretching experiments confirmed that DOX-treated NB4 cells were stiffer than the untreated cells. The structural parameters extracted from the model and the confocal images indicated significant change in actin network after DOX treatment.

conclusionThe proposed DEP method combined with actin cytoskeleton modeling is a simple engineering tool to characterize the mechanical properties of cells.

Indexed as

Mechanical PhenomenaModels, BiologicalActin CytoskeletonBiomechanical PhenomenaCell Line, TumorCost-Benefit AnalysisElectrophoresisHumansMechanotransduction, CellularStress, MechanicalCell stretchingCytoskeleton modelDielectrophoresisMechanical propertyOptical tweezers

Identifiers

PMID28376803
PMCPMC5381122
OpenAlexW2605362731

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.