ArticleBMC genomics2017
Construction of a high-density genetic map and the X/Y sex-determining gene mapping in spinach based on large-scale markers developed by specific-locus amplified fragment sequencing (SLAF-seq).
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Gibberellin Disrupts Hormonal Homeostasis and Anther Integrity to Trigger Sex Reversal in Spinach.International journal of molecular sciences · 2025Article
- Genome-Wide Identification and Characterization of MYB Gene Family and Analysis of Its Sex-Biased Expression Pattern inInternational journal of molecular sciences · 2024Article
- Evolution of plant sex and molecular mechanisms underlying plants sex separation.Forestry research · 2023Review
- Identification of Sex Differentiation-Related microRNAs in Spinach Female and Male Flower.International journal of molecular sciences · 2022Article
- Identification of structural variation and polymorphisms of a sex co-segregating scaffold in spinach.Plant reproduction · 2022Article
- Quantitative Trait Locus Mapping and Identification of Candidate Genes Controlling Bolting in Spinach (Frontiers in plant science · 2022Article
- High resolution mapping and candidate gene identification of downy mildew race 16 resistance in spinach.BMC genomics · 2021Article
- Quantitative trait loci (QTL) analysis of leaf related traits in spinach (Spinacia oleracea L.).BMC plant biology · 2021Article
- Fine mapping and molecular marker development of the Fs gene controlling fruit spines in spinach (Spinacia oleracea L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Construction of high-density genetic maps defined sex determination region of the Y chromosome in spinach.Molecular genetics and genomics : MGG · 2021Article
- Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei).BMC genomics · 2020Article
- Comparative transcriptome analysis of male and female flowers in Spinacia oleracea L.BMC genomics · 2020Article
- Identification of candidate genes for key fibre-related QTLs and derivation of favourable alleles in Gossypium hirsutum recombinant inbred lines with G. barbadense introgressions.Plant biotechnology journal · 2020Article
- Construction, characteristics and high throughput molecular screening methodologies in some special breeding populations: a horticultural perspective.Journal of genetics · 2019Review
- QTL mapping pod dehiscence resistance in soybean (Glycine max L. Merr.) using specific-locus amplified fragment sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- High-density genetic map construction and QTL mapping of first flower node in pepper (Capsicum annuum L.).BMC plant biology · 2019Article
- Construction of a High-Density Genetic Map and Analysis of Seed-Related Traits Using Specific Length Amplified Fragment Sequencing forFrontiers in plant science · 2019Article
- Fine mapping and candidate gene screening of the downy mildew resistance gene RPF1 in Spinach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCultivated spinach (Spinacia oleracea L.) is one of the most widely cultivated types of leafy vegetable in the world, and it has a high nutritional value. Spinach is also an ideal plant for investigating the mechanism of sex determination because it is a dioecious species with separate male and female plants. Some reports on the sex labeling and localization of spinach in the study of molecular markers have surfaced. However, there have only been two reports completed on the genetic map of spinach. The lack of rich and reliable molecular markers and the shortage of high-density linkage maps are important constraints in spinach research work. In this study, a high-density genetic map of spinach based on the Specific-locus Amplified Fragment Sequencing (SLAF-seq) technique was constructed; the sex-determining gene was also finely mapped.
resultsThrough bio-information analysis, 50.75 Gb of data in total was obtained, including 207.58 million paired-end reads. Finally, 145,456 high-quality SLAF markers were obtained, with 27,800 polymorphic markers and 4080 SLAF markers were finally mapped onto the genetic map after linkage analysis. The map spanned 1,125.97 cM with an average distance of 0.31 cM between the adjacent marker loci. It was divided into 6 linkage groups corresponding to the number of spinach chromosomes. Besides, the combination of Bulked Segregation Analysis (BSA) with SLAF-seq technology(super-BSA) was employed to generate the linkage markers with the sex-determining gene. Combined with the high-density genetic map of spinach, the sex-determining gene X/Y was located at the position of the linkage group (LG) 4 (66.98 cM-69.72 cM and 75.48 cM-92.96 cM), which may be the ideal region for the sex-determining gene.
conclusionsA high-density genetic map of spinach based on the SLAF-seq technique was constructed with a backcross (BC
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