ArticleMolecular and cellular biology1988
Stably transmitted triple-promoter retroviral vectors and their use in transformation of primary mammalian cells.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- The Francisella O-antigen mediates survival in the macrophage cytosol via autophagy avoidance.Cellular microbiology · 2014Article
- Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages.Journal of immunological methods · 2008Article
- Recombinant retrovirus vectors for the expression of MHC class II heterodimers.Surgery today · 1999Article
- Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy.Journal of virology · 1996Article
- Retroviral vectors. From laboratory tools to molecular medicine.Molecular biotechnology · 1996Review
- Complementation cell lines for viral vectors to be used in gene therapy.Cytotechnology · 1995Article
- Appropriate in vivo expression of a muscle-specific promoter by using avian retroviral vectors for gene transfer [corrected].Journal of virology · 1992Article
- Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Retroviral vectors containing putative internal ribosome entry sites: development of a polycistronic gene transfer system and applications to human gene therapy.Nucleic acids research · 1992Article
- The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.Molecular and cellular biology · 1991Article
- Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs.Journal of virology · 1991Article
- Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.Molecular and cellular biology · 1991Article
- Development of a retroviral vector for inducible expression of transforming growth factor beta 1.Journal of virology · 1990Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retroviral vectors were constructed which coexpressed three inserted genes from independent transcriptional promoters in singly infected cells. Several such triple-promoter vectors were constructed with various combinations of oncogenes and selectable drug resistance genes. All expressed three mRNAs of the expected size in infected cells. One vector expressing the v-Ha-ras, v-myc, and neo genes was characterized in detail. This retrovirus did not undergo rearrangement during the process of infection, as judged by Southern analysis, and infection of primary rat embryo fibroblasts demonstrated that ras-myc-cotransformed cells could be selected in G418. This demonstration that retroviral vectors can be used to express three cistrons independently increases their value as gene transfer vehicles, particularly for studies involving oncogene cooperation in primary cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.