Evidence map›Paper›PMID 28376366›Full record

ArticleBiomaterials2017

Enzymatically crosslinked silk-hyaluronic acid hydrogels.

Nicole R Raia, Benjamin P Partlow, Meghan McGill, Erica Palma Kimmerling, Chiara E Ghezzi, David L Kaplan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.

0numbers the graph read from it
0cells of the map it votes in
93citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

93 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. 3D Printing-Based Hydrogel Dressings for Wound Healing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  20. Review

33 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nicole R RaiaDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
Benjamin P PartlowDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
Meghan McGillDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
Erica Palma KimmerlingDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
Chiara E GhezziDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA. Electronic address: david.kaplan@tufts.edu.

Funding

Translational Bioreactors - Engineering Complex TissuesP41EB002520 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI KAPLAN, DAVID L. · 2004 to 2018
$16.7M
Injectable Gels for Tissue AugmentationR01EB021264 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI HOUSE, MICHAEL DOUGLAS, KAPLAN, DAVID L. · 2016 to 2019
$1.4M
NIBIB NIH HHS P41 EB002520NIBIB NIH HHS R01 EB021264
6 · The paper itself

Abstract

In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues. The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization. Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly. To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity. The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration. In addition, the hydrogels were characterized by enzymatic degradation and for cytotoxicity. Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization. These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.

Indexed as

AnimalsBiocompatible MaterialsBombyxCells, CulturedCross-Linking ReagentsElasticityHumansHyaluronic AcidHydrogelsMaterials TestingMesenchymal Stem CellsRheologySilkTissue ScaffoldsBiocompatible MaterialsCross-Linking ReagentsHyaluronic AcidHydrogelsSilkBiomaterialsEnzymatic crosslinkingHydrogel blendsPolymer compositesTemporal stiffening

Identifiers

PMID28376366
PMCPMC5479139

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.