ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2017
Tyrosine Phosphorylation of an Actin-Binding Protein Girdin Specifically Marks Tuft Cells in Human and Mouse Gut.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells.The Journal of cell biology · 2024Article
- Colonic Tuft Cells: The Less-Recognized Therapeutic Targets in Inflammatory Bowel Disease and Colorectal Cancer.International journal of molecular sciences · 2024Review
- Intestinal Tuft Cells: Morphology, Function, and Implications for Human Health.Annual review of physiology · 2024Review
- Novel protocol to observe the intestinal tuft cell using transmission electron microscopy.Biology open · 2022Article
- Single-Cell Transcriptomics Reveals a Conserved Metaplasia Program in Pancreatic Injury.Gastroenterology · 2022Article
- Tuft Cells and Their Role in Intestinal Diseases.Frontiers in immunology · 2022Review
- Luminal Chemosensory Cells in the Small Intestine.Nutrients · 2021Review
- Tuning tuft cells: new ligands and effector functions reveal tissue-specific function.Current opinion in immunology · 2021Review
- Distribution of duodenal tuft cells is altered in pediatric patients with acute and chronic enteropathy.Biomedical research (Tokyo, Japan) · 2020Article
- Regulation of immune responses by tuft cells.Nature reviews. Immunology · 2019Review
- Single-Cell Imaging of Metastatic Potential of Cancer Cells.iScience · 2018Article
- Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections.Journal of visualized experiments : JoVE · 2018Article
- Luminal chemosensing in the gastroduodenal mucosa.Current opinion in gastroenterology · 2017Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuft cells (TCs) are minor components of gastrointestinal epithelia, characterized by apical tufts and spool-shaped somas. The lack of reliable TC-markers has hindered the elucidation of its role. We developed site-specific and phosphorylation-status-specific antibodies against Girdin at tyrosine-1798 (pY1798) and found pY1798 immunostaining of mouse jejunum clearly depicted epithelial cells closely resembling TCs. This study aimed to validate pY1798 as a TC-marker. Double-fluorescence staining of intestines was performed with pY1798 and known TC-markers, for example, hematopoietic-prostaglandin-D-synthase (HPGDS), or doublecortin-like kinase 1 (DCLK1). Odds ratios (ORs) were calculated from cell counts to determine whether two markers were attracting (OR<1) or repelling (OR>1). In consequence, pY1798 signals strongly attracted those of known TC-markers. ORs for HPGDS in mouse stomach, small intestine, and colon were 0 for all, and 0.08 for DCLK1 in human small intestine. pY1798-positive cells in jejunum were distinct from other minor epithelial cells, including goblet, Paneth, and neuroendocrine cells. Thus, pY1798 was validated as a TC-marker. Interestingly, apoptosis inducers significantly increased relative TC frequencies despite the absence of proliferation at baseline. In conclusion, pY1798 is a novel TC-marker. Selective tyrosine phosphorylation and possible resistance to apoptosis inducers implied the activation of certain kinase(s) in TCs, which may become a clue to elucidate the enigmatic roles of TCs. .
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Registered trials
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