Evidence map›Paper›PMID 28373698›Full record

ArticleScientific reports2017

The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.

Emma L Hesketh, Yulia Meshcheriakova, Rebecca F Thompson, George P Lomonossoff, Neil A Ranson

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
7.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

  1. Review
  2. Review
  3. Plant-based biopharmaceutical engineering.Nature reviews bioengineering · 2023
    Review
  4. Article
  5. Materials advances · 2021
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Encapsidation of Viral RNA inJournal of virology · 2019
    Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Emma L HeskethAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK. e.l.hesketh@leeds.ac.uk.
Yulia MeshcheriakovaDepartment of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK.
Rebecca F ThompsonAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.
George P LomonossoffDepartment of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK.
Neil A RansonAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK. n.a.ranson@leeds.ac.uk.ORCID http://orcid.org/0000-0002-3640-5275
University of Leeds · GBJohn Innes Centre · GB

Funding

Biotechnology and Biological Sciences Research Council BBS/E/J/00000166Wellcome Trust 090932
6 · The paper itself

Abstract

Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.

Indexed as

Genome, ViralCapsidCapsid ProteinsComovirusCryoelectron MicroscopyImage Processing, Computer-AssistedImaging, Three-DimensionalProtein SubunitsRNA, ViralVirionCapsid ProteinsProtein SubunitsRNA, Viral

Identifiers

PMID28373698
PMCPMC5428714
OpenAlexW2603761583

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.