Evidence map›Paper›PMID 28370550›Full record

ReviewProtein science : a publication of the Protein Society2017

Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence.

Yazan Alhadid, SangYoon Chung, Eitan Lerner, Dylan J Taatjes, Sergei Borukhov, Shimon Weiss

Open access · bronzeAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Protein science : a publication of the Protein Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 17 citations in OpenAlex.

  1. Understanding the impact ofFrontiers in molecular biosciences · 2024
    Review
  2. Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022
    Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yazan AlhadidInterdepartmental Program in Molecular, Cellular, and Integrative Physiology, University of California, Los Angeles, California, 90095.
SangYoon ChungDepartment of Chemistry & Biochemistry, University of California, Los Angeles, California, 90095.
Eitan LernerDepartment of Chemistry & Biochemistry, University of California, Los Angeles, California, 90095.
Dylan J TaatjesDepartment of Chemistry & Biochemistry, University of Colorado, Boulder, Colorado, 80303.
Sergei BorukhovRowan University School of Osteopathic Medicine, Stratford, New Jersey, 08084.
Shimon WeissInterdepartmental Program in Molecular, Cellular, and Integrative Physiology, University of California, Los Angeles, California, 90095.
University of California, Los Angeles · USRowan University · USUniversity of Colorado Boulder · US

Funding

Single Molecule Studies of Prokaryotic and Eukaryotic Transcription MachineriesR01GM069709 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2005 to 2012
$2.9M
Structural dynamics of RNAP-promoter complex in late transcription initiationR01GM130942 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2019 to 2022
$2.2M
Single-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0M
NIGMS NIH HHS R01 GM069709NIGMS NIH HHS R01 GM095904NIGMS NIH HHS R01 GM130942
6 · The paper itself

Abstract

Over the past decade, fluorescence-based single-molecule studies significantly contributed to characterizing the mechanism of RNA polymerase at different steps in transcription, especially in transcription initiation. Transcription by bacterial DNA-dependent RNA polymerase is a multistep process that uses genomic DNA to synthesize complementary RNA molecules. Transcription initiation is a highly regulated step in E. coli, but it has been challenging to study its mechanism because of its stochasticity and complexity. In this review, we describe how single-molecule approaches have contributed to our understanding of transcription and have uncovered mechanistic details that were not observed in conventional assays because of ensemble averaging.

Indexed as

DNA, BacterialDNA-Directed DNA PolymeraseEscherichia coliEscherichia coli ProteinsFluorescenceRNA, BacterialTranscription Initiation, GeneticDNA, BacterialDNA-Directed DNA PolymeraseEscherichia coli ProteinsRNA, Bacterialalternating laser excitationFRETRNA polymerasesingle moleculetranscription

Identifiers

PMID28370550
PMCPMC5477543
OpenAlexW2602282391

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.