Evidence map›Paper›PMID 28370547›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2017

Real-Time Monitoring of Enzyme-Catalysed Reactions using Deep UV Resonance Raman Spectroscopy.

Chloe Westley, Heidi Fisk, Yun Xu, Katherine A Hollywood, Andrew J Carnell, Jason Micklefield, Nicholas J Turner, Royston Goodacre

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. New assay method based on Raman spectroscopy for enzymes reacting with gaseous substrates.Protein science : a publication of the Protein Society · 2019
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Chloe WestleySchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.
Heidi FiskSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.
Yun XuSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.ORCID http://orcid.org/0000-0003-3228-5111
Katherine A HollywoodSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.ORCID http://orcid.org/0000-0002-7028-047X
Andrew J CarnellDepartment of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK.ORCID http://orcid.org/0000-0002-6353-403X
Jason MicklefieldSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.ORCID http://orcid.org/0000-0001-8951-4873
Nicholas J TurnerSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.ORCID http://orcid.org/0000-0002-8708-0781
Royston GoodacreSchool of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess street, Manchester, M1 7DN, UK.ORCID http://orcid.org/0000-0003-2230-645X
University of Manchester · GBUniversity of Liverpool · GB

Funding

Biotechnology and Biological Sciences Research Council BB/K00199X/1Biotechnology and Biological Sciences Research Council BB/M017702/1
6 · The paper itself

Abstract

For enzyme-catalysed biotransformations, continuous in situ detection methods minimise the need for sample manipulation, ultimately leading to more accurate real-time kinetic determinations of substrate(s) and product(s). We have established for the first time an on-line, real-time quantitative approach to monitor simultaneously multiple biotransformations based on UV resonance Raman (UVRR) spectroscopy. To exemplify the generality and versatility of this approach, multiple substrates and enzyme systems were used involving nitrile hydratase (NHase) and xanthine oxidase (XO), both of which are of industrial and biological significance, and incorporate multistep enzymatic conversions. Multivariate data analysis of the UVRR spectra, involving multivariate curve resolution-alternating least squares (MCR-ALS), was employed to effect absolute quantification of substrate(s) and product(s); repeated benchmarking of UVRR combined with MCR-ALS by HPLC confirmed excellent reproducibility.

Indexed as

BiocatalysisChromatography, High Pressure LiquidHydro-LyasesLeast-Squares AnalysisSpectrophotometry, UltravioletSpectrum Analysis, RamanSubstrate SpecificityXanthine OxidaseHydro-Lyasesnitrile hydrataseXanthine Oxidasebiotransformationson-line monitoringquantificationreaction monitoringUV resonance Raman (UVRR) spectroscopy

Identifiers

PMID28370547
PMCPMC5488198
OpenAlexW2602989945

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.