ArticleJournal of experimental botany2017
Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Silencing an ATP-Dependent Caseinolytic Protease Proteolytic Subunit Gene Enhances the Resistance of Rice toInternational journal of molecular sciences · 2024Article
- AmCBF1 activates the expression of GhClpR1 to mediate dark-green leaves in cotton (Gossypium hirsutum).Plant cell reports · 2024Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Proteolytic regulation of mitochondrial oxidative phosphorylation components in plants.Biochemical Society transactions · 2022Review
- Comprehensive analysis of plastid gene expression during fruit development and ripening of kiwifruit.Plant cell reports · 2022Article
- Gene duplication and rate variation in the evolution of plastid ACCase and Clp genes in angiosperms.Molecular phylogenetics and evolution · 2022Article
- Rapid sequence evolution is associated with genetic incompatibilities in the plastid Clp complex.Plant molecular biology · 2022Article
- A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- The availability of neither D2 nor CP43 limits the biogenesis of photosystem II in tobacco.Plant physiology · 2021Article
- Knockdown of the plastid-encoded acetyl-CoA carboxylase gene uncovers functions in metabolism and development.Plant physiology · 2021Article
- The Intron Retention VariantFrontiers in plant science · 2021Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2021Article
- A Bivariate Mapping Model Identifies Major Covariation QTLs for Biomass Allocation Between Leaf and Stem Growth ofFrontiers in genetics · 2021Article
- Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes.Plant physiology · 2020Article
- Co-Suppression ofPlants (Basel, Switzerland) · 2020Article
- Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins.Journal of experimental botany · 2019Article
- Co-suppression of NbClpC1 and NbClpC2 alters plant morphology with changed hormone levels in Nicotiana benthamiana.Plant cell reports · 2019Article
- Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity.Molecular & cellular proteomics : MCP · 2019Article
- A High-Throughput Interbacterial Competition Screen Identifies ClpAP in Enhancing Recipient Susceptibility to Type VI Secretion System-Mediated Attack byFrontiers in microbiology · 2019Article
- Consequences of the loss of catalytic triads in chloroplast CLPPR protease core complexes in vivo.Plant direct · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein degradation in chloroplasts is carried out by a set of proteases that eliminate misfolded, damaged, or superfluous proteins. The ATP-dependent caseinolytic protease (Clp) is the most complex protease in plastids and has been implicated mainly in stromal protein degradation. In contrast, FtsH, a thylakoid membrane-associated metalloprotease, is believed to participate mainly in the degradation of thylakoidal proteins. To determine the role of specific Clp and FtsH subunits in plant growth and development, RNAi lines targeting at least one subunit of each Clp ring and FtsH were generated in tobacco. In addition, mutation of the translation initiation codon was employed to down-regulate expression of the plastid-encoded ClpP1 subunit. These protease lines cover a broad range of reductions at the transcript and protein levels of the targeted genes. A wide spectrum of phenotypes was obtained, including pigment deficiency, alterations in leaf development, leaf variegations, and impaired photosynthesis. When knock-down lines for the different protease subunits were compared, both common and specific phenotypes were observed, suggesting distinct functions of at least some subunits. Our work provides a well-characterized collection of knock-down lines for plastid proteases in tobacco and reveals the importance of the Clp protease in physiology and plant development.
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