ArticlePLoS pathogens2017
Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- A Dual-Receptor System MediatesJournal of agricultural and food chemistry · 2026Article
- Sugars, Lipids and More: New Insights Into Plant Carbon Sources During Plant-Microbe Interactions.Plant, cell & environment · 2025Review
- Thermal shift assay to identify ligands for bacterial sensor proteins.FEMS microbiology reviews · 2025Review
- Stenotrophomonas maltophilia uses a c-di-GMP module to sense the mammalian body temperature during infection.PLoS pathogens · 2024Article
- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- A Stenotrophomonas maltophilia TetR-Like Transcriptional Regulator Involved in Fatty Acid Metabolism Is Controlled by Quorum Sensing Signals.Applied and environmental microbiology · 2023Article
- Biofilm-Forming Ability of Phytopathogenic Bacteria: A Review of its Involvement in Plant Stress.Plants (Basel, Switzerland) · 2023Review
- DSF inactivator RpfB homologous FadD upregulated in Bradyrhizobium japonicum under iron limiting conditions.Scientific reports · 2023Article
- Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming.mBio · 2022Article
- Comparative genome analysis unravels pathogenicity of Xanthomonas albilineans causing sugarcane leaf scald disease.BMC genomics · 2022Article
- Review
- Article
- BDSF Is a Degradation-Prone Quorum-Sensing Signal Detected by the Histidine Kinase RpfC ofApplied and environmental microbiology · 2022Article
- TheApplied and environmental microbiology · 2022Review
- Integration of DSF and Temperature Signals for RpfC/RpfG Two-Component System Modulating Protease Production in Stenotrophomonas maltophilia FF11.Current microbiology · 2022Article
- Multidrug-resistant Acinetobacter baumannii as an emerging concern in hospitals.Molecular biology reports · 2021Review
- Clp is a "busy" transcription factor in the bacterial warrior,Computational and structural biotechnology journal · 2021Review
- The Roles of Microbial Cell-Cell Chemical Communication Systems in the Modulation of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2020Review
- RpoN1 and RpoN2 play different regulatory roles in virulence traits, flagellar biosynthesis, and basal metabolism in Xanthomonas campestris.Molecular plant pathology · 2020Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As well as their importance to nutrition, fatty acids (FA) represent a unique group of quorum sensing chemicals that modulate the behavior of bacterial population in virulence. However, the way in which full-length, membrane-bound receptors biochemically detect FA remains unclear. Here, we provide genetic, enzymological and biophysical evidences to demonstrate that in the phytopathogenic bacterium Xanthomonas campestris pv. campestris, a medium-chain FA diffusible signal factor (DSF) binds directly to the N-terminal, 22 amino acid-length sensor region of a receptor histidine kinase (HK), RpfC. The binding event remarkably activates RpfC autokinase activity by causing an allosteric change associated with the dimerization and histidine phosphotransfer (DHp) and catalytic ATP-binding (CA) domains. Six residues were found essential for sensing DSF, especially those located in the region adjoining to the inner membrane of cells. Disrupting direct DSF-RpfC interaction caused deficiency in bacterial virulence and biofilm development. In addition, two amino acids within the juxtamembrane domain of RpfC, Leu172 and Ala178, are involved in the autoinhibition of the RpfC kinase activity. Replacements of them caused constitutive activation of RpfC-mediated signaling regardless of DSF stimulation. Therefore, our results revealed a biochemical mechanism whereby FA activates bacterial HK in an allosteric manner, which will assist in future studies on the specificity of FA-HK recognition during bacterial virulence regulation and cell-cell communication.
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