Evidence map›Paper›PMID 28369109›Full record

ArticlePloS one2017

Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors.

Yang Sun, Wenwen Guo, Fen Wang, Chunjun Zhan, Yankun Yang, Xiuxia Liu, Zhonghu Bai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yang SunNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Wenwen GuoNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Fen WangNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Chunjun ZhanNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Yankun YangNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Xiuxia LiuNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Zhonghu BaiNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Jiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Corynebacterium glutamicum (C. glutamicum) is a favorable host cell for the production of recombinant proteins, such as important enzymes and pharmaceutical proteins, due to its excellent potential advantages. Herein, we sought to systematically explore the influence of recombinant protein expression on the transcription and metabolism of C. glutamicum. Two C. glutamicum strains, the wild-type strain and an engineered strain expressing enhanced green fluorescent protein (EGFP), were cultured in parallel in 5-L bioreactors to study the change in metabolism in the process of EGFP expression. The results revealed that EGFP expression had great effects on the growth and metabolism of C. glutamicum and contributed to metabolism-like anaerobic conditions as follows: glycolysis was enhanced, the TCA cycle was shunted, and Glu, Val, Met, lactate and acetate were accumulated to produce sufficient ATP for EGFP production and transfer. Many differentially expressed genes related to ribosomal protein, transcriptional regulators, and energy metabolism were found to be expressed in the presence of EGFP, laying the foundation for identifying genomic loci to change the flow of the host cell metabolism to improve the ability of expressing foreign proteins in C. glutamicum.

Indexed as

BioreactorsAdenosine TriphosphateAmino AcidsBiomassChromatography, High Pressure LiquidCorynebacterium glutamicumFermentationGene Expression ProfilingGene OntologyGlucoseGreen Fluorescent ProteinsMetabolic EngineeringReal-Time Polymerase Chain ReactionRecombinant ProteinsAdenosine TriphosphateAmino Acidsenhanced green fluorescent proteinGlucoseGreen Fluorescent ProteinsRecombinant Proteins

Identifiers

PMID28369109
PMCPMC5378358
OpenAlexW2604845239

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.