ArticlePloS one2017
Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 24 citations in OpenAlex.
- Ancient Species Diversity and Niche Adaptation in Tannerella and Porphyromonas Revealed Through Pangenomics.Genome biology and evolution · 2026Article
- Differential Gene Expression ofInternational journal of molecular sciences · 2025Article
- Probiotics and amelioration of periodontitis: significant roles ofFrontiers in microbiology · 2025Article
- Type 1 fimbrial phase variation in multidrug-resistant asymptomatic uropathogenic Escherichia coli clinical isolates upon adherence to HTB-4 cells.Folia microbiologica · 2024Article
- Phylogenomic analysis of theFrontiers in microbiology · 2023Article
- Contribution of -Omics Technologies in the Study ofInternational journal of molecular sciences · 2022Review
- Peptidomimetics as Potential Anti-Virulence Drugs Against Resistant Bacterial Pathogens.Frontiers in microbiology · 2022Review
- Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems.Microorganisms · 2021Review
- Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model.Nutrients · 2020Article
- Biofilm architecture: An emerging synthetic biology target.Synthetic and systems biotechnology · 2020Review
- Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells.Molecular medicine reports · 2020Article
- Porphyromonas gingivalis genes conferring fitness in a tobacco-rich environment.Molecular oral microbiology · 2020Article
- Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.BMC microbiology · 2019Article
- Gene expression of Porphyromonas gingivalis ATCC 33277 when growing in an in vitro multispecies biofilm.PloS one · 2019Article
- Article
- A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division inPlant physiology · 2017Article
- Indonesian Mangosteen Fruit (Contemporary clinical dentistryArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivePorphyromonas gingivalis is a keystone pathogen in the onset and progression of periodontitis. Its pathogenicity has been related to its presence and survival within the subgingival biofilm. The aim of the present study was to compare the genome-wide transcription activities of P. gingivalis in biofilm and in planktonic growth, using microarray technology. MATERIAL AND
methodsP. gingivalis ATCC 33277 was incubated in multi-well culture plates at 37°C for 96 hours under anaerobic conditions using an in vitro static model to develop both the planktonic and biofilm states (the latter over sterile ceramic calcium hydroxyapatite discs). The biofilm development was monitored by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). After incubation, the bacterial cells were harvested and total RNA was extracted and purified. Three biological replicates for each cell state were independently hybridized for transcriptomic comparisons. A linear model was used for determining differentially expressed genes and reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to confirm differential expression. The filtering criteria of ≥ ±2 change in gene expression and significance p-values of <0.05 were selected.
resultsA total of 92 out of 1,909 genes (4.8%) were differentially expressed by P. gingivalis growing in biofilm compared to planktonic. The 54 up-regulated genes in biofilm growth were mainly related to cell envelope, transport, and binding or outer membranes proteins. Thirty-eight showed decreased expression, mainly genes related to transposases or oxidative stress.
conclusionThe adaptive response of P. gingivalis in biofilm growth demonstrated a differential gene expression.
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