Evidence map›Paper›PMID 28368370›Full record

ArticleGene therapy2017

MnSOD mediated by HSV vectors in the periaqueductal gray suppresses morphine withdrawal in rats.

T Iida, H Yi, S Liu, D Ikegami, W Zheng, Q Liu, K Takahashi, Y Kashiwagi, W F Goins, J C Glorioso and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

T IidaDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
H YiDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
S LiuDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
D IkegamiDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
W ZhengDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
Q LiuDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
K TakahashiDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
Y KashiwagiDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
W F GoinsDepartment of Microbiology and Molecular Genetics, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
J C GloriosoDepartment of Microbiology and Molecular Genetics, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
S HaoDepartment of Anesthesiology, University of Miami Miller School of Medicine, Miami, FL, USA.
University of Miami · USUniversity of Pittsburgh · US

Funding

Neuropathic mechanisms and gene therapy on opioid dependenceR01DA034749 · NIDA · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI HAO, SHUANGLIN · 2014 to 2018
$1.7M
HIV and NRTI's-induced painful pathogenic mechanisms and gene therapyR01NS066792 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAO, SHUANGLIN · 2010 to 2014
$1.6M
Transgene for morphine tolerance and withdrawalR03DA020078 · NIDA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAO, SHUANGLIN · 2005 to 2005
$77k
NIDA NIH HHS R01 DA034749NIDA NIH HHS R03 DA020078NINDS NIH HHS R01 NS066792
6 · The paper itself

Abstract

Morphine appears to be the most active metabolite of heroin; therefore, the effects of morphine are important in understanding the ramifications of heroin abuse. Opioid physical dependence (withdrawal response) may have very long-lasting effects on the motivation for reward, including the incubation of cue-induced drug-seeking behavior. However, the exact mechanisms of morphine withdrawal (MW) are not clear yet, and its treatment remains elusive. Periaqueductal gray (PAG) is one of the important sites in the pathogenesis of MW. Here, we used recombinant herpes simplex virus (HSV) vectors that encode the sod2 gene expressing manganese superoxide dismutase (MnSOD) to evaluate its therapeutic potential in MW. Microinjection of HSV vectors expressing MnSOD into the PAG reduced the MW syndrome. MnSOD vectors suppressed the upregulated mitochondrial superoxide, and endoplasmic reticulum stress markers (glucose-related protein 78 (GRP78) and activating transcription factor 6 alpha (ATF6α)) in the PAG induced by MW. Immunostaining showed that mitochondrial superoxide, GRP78 and ATF6α were colocalized with neuronal nuclei (a neuronal-specific marker), suggesting that they are located in the neurons in the PAG. These results suggest that overexpression of MnSOD by HSV vectors may relieve opioid dependence. This study may provide a novel therapeutic approach to morphine physical withdrawal response.

Indexed as

Genetic TherapyActivating Transcription Factor 6AnimalsEndoplasmic Reticulum Chaperone BiPGenetic VectorsHeat-Shock ProteinsMaleMorphinePeriaqueductal GrayRatsRats, Sprague-DawleySimplexvirusSubstance Withdrawal SyndromeSuperoxide DismutaseActivating Transcription Factor 6Atf6 protein, ratEndoplasmic Reticulum Chaperone BiPGRP78 protein, ratHeat-Shock ProteinsMorphineSuperoxide Dismutase

Identifiers

PMID28368370
PMCPMC9870211
OpenAlexW2605050827

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.