ArticleScientific reports2017
CRISPR/Cas9-mediated efficient genome editing via blastospore-based transformation in entomopathogenic fungus Beauveria bassiana.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 79 citations in OpenAlex.
- Review
- CRISPR/Cas9-assisted gene editing reveals that EgPKS, a polyketide synthase, is required for the biosynthesis of preussomerins in Edenia gomezpompae SV2.World journal of microbiology & biotechnology · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Microbial alternatives for sustainable insecticide use, a Canadian perspective.Sustainable microbiology · 2025Article
- CRISPR-Cas9-mediated enhancement of Beauveria bassiana virulence with overproduction of oosporein.Fungal biology and biotechnology · 2024Article
- A user-friendly CRISPR/Cas9 system for mutagenesis of Neurospora crassa.Scientific reports · 2024Article
- Expression of microbial lipase in filamentous fungus3 Biotech · 2024Review
- Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 inPlants (Basel, Switzerland) · 2024Article
- Comparing Fungal Sensitivity to Isothiocyanate Products on DifferentPlants (Basel, Switzerland) · 2024Article
- General aspects, host interaction, and application ofFrontiers in fungal biology · 2024Review
- Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality.Frontiers in microbiology · 2024Review
- Analysis of the virulence, infection process, and extracellular enzyme activities ofVirulence · 2023Article
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- Beauveria bassiana rewires molecular mechanisms related to growth and defense in tomato.Journal of experimental botany · 2023Article
- Establishment of a CRISPR/Cas9-Mediated Efficient Knockout System ofJournal of fungi (Basel, Switzerland) · 2023Article
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
- Use of CRISPR-Cas tools to engineer Trichoderma species.Microbial biotechnology · 2022Review
- Targeted Gene Mutations in the Forest PathogenPlants (Basel, Switzerland) · 2022Article
- Development of versatile and efficient genetic tools for the marine-derived fungus Aspergillus terreus RA2905.Current genetics · 2022Article
- Delineation of mechanistic approaches of rhizosphere microorganisms facilitated plant health and resilience under challenging conditions.3 Biotech · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beauveria bassiana is an environmentally friendly alternative to chemical insecticides against various agricultural insect pests and vectors of human diseases. However, its application has been limited due to slow kill and sensitivity to abiotic stresses. Understanding of the molecular pathogenesis and physiological characteristics would facilitate improvement of the fungal performance. Loss-of-function mutagenesis is the most powerful tool to characterize gene functions, but it is hampered by the low rate of homologous recombination and the limited availability of selectable markers. Here, by combining the use of uridine auxotrophy as recipient and donor DNAs harboring auxotrophic complementation gene ura5 as a selectable marker with the blastospore-based transformation system, we established a highly efficient, low false-positive background and cost-effective CRISPR/Cas9-mediated gene editing system in B. bassiana. This system has been demonstrated as a simple and powerful tool for targeted gene knock-out and/or knock-in in B. bassiana in a single gene disruption. We further demonstrated that our system allows simultaneous disruption of multiple genes via homology-directed repair in a single transformation. This technology will allow us to study functionally redundant genes and holds significant potential to greatly accelerate functional genomics studies of B. bassiana.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.