ArticleScientific reports2017
Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
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- Selection and evaluation of reference genes for quantitative real-time polymerase chain reaction normalization in Pieris melete (Lepidoptera, Pieridae).Journal of insect science (Online) · 2025Article
- A knockdown in the expression of a male-specific aminopeptidase impedes the male reproductive success of the mosquitoPhysiological entomology · 2025Article
- The Selection and Validation of Reference Genes for RT-qPCR Analysis of the Predatory Natural EnemyInsects · 2024Article
- Stability and suitability of housekeeping genes in phlebotomine sand flies.Scientific reports · 2024Article
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- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach ParasitoidInsects · 2024Article
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
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- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
- Evaluation of Reference Genes for Quantitative Reverse Transcription Polymerase Chain Reaction inInsects · 2021Article
- Engineered sex ratio distortion by X-shredding in the global agricultural pest Ceratitis capitata.BMC biology · 2021Article
- Nanopore long-read RNA-seq and absolute quantification delineate transcription dynamics in early embryo development of an insect pest.Scientific reports · 2021Article
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- Anosmic flies: what Orco silencing does to olive fruit flies.BMC genetics · 2020Article
- Effect of Nitrogen Sources on Omega-3 Polyunsaturated Fatty Acid Biosynthesis and Gene Expression inMarine drugs · 2020Article
- Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).Scientific reports · 2020Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review.Frontiers in physiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time quantitative-PCR has been a priceless tool for gene expression analyses. The reaction, however, needs proper normalization with the use of housekeeping genes (HKGs), whose expression remains stable throughout the experimental conditions. Often, the combination of several genes is required for accurate normalization. Most importantly, there are no universal HKGs which can be used since their expression varies among different organisms, tissues or experimental conditions. In the present study, nine common HKGs (RPL19, tbp, ubx, GAPDH, α-TUB, β-TUB, 14-3-3zeta, RPE and actin3) are evaluated in thirteen different body parts, developmental stages and reproductive and olfactory tissues of two insects of agricultural importance, the medfly and the olive fly. Three software programs based on different algorithms were used (geNorm, NormFinder and BestKeeper) and gave different ranking of HKG stabilities. This confirms once again that the stability of common HKGs should not be taken for granted and demonstrates the caution that is needed in the choice of the appropriate HKGs. Finally, by estimating the average of a standard score of the stability values resulted by the three programs we were able to provide a useful consensus key for the choice of the best HKG combination in various tissues of the two insects.
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