ArticleScientific reports2017
Riboflavin deficiency induces a significant change in proteomic profiles in HepG2 cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Endoplasmic reticulum stress as a mechanistic link between nutrition and inflammatory bowel disease.Frontiers in nutrition · 2026Review
- Increased production of riboflavin in Ashbya gossypii by endoplasmic reticulum stresses.Archives of microbiology · 2025Article
- Interruptible demyelination in avian riboflavin deficient neuropathy.Cell & bioscience · 2024Article
- Effects of riboflavin deficiency and high dietary fat on hepatic lipid accumulation: a synergetic action in the development of non-alcoholic fatty liver disease.Nutrition & metabolism · 2024Article
- From Metabolism to Vitality: Uncovering Riboflavin's Importance in Poultry Nutrition.Animals : an open access journal from MDPI · 2023Review
- Novel Therapeutic Strategies Exploiting the Unique Properties of Neuroendocrine Neoplasms.Cancers · 2023Article
- Vitamin combination promotes ex vivo expansion of NK-92 cells by reprogramming glucose metabolism.Bioresources and bioprocessing · 2022Article
- Riboflavin (Vitamin B2) Deficiency Induces Apoptosis Mediated by Endoplasmic Reticulum Stress and the CHOP Pathway in HepG2 Cells.Nutrients · 2022Article
- Riboflavin Stabilizes Abasic, Oxidized G-Quadruplex Structures.Biochemistry · 2022Article
- Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells.International journal of molecular sciences · 2021Article
- Strategies to Increase the Production of Biosynthetic Riboflavin.Molecular biotechnology · 2021Review
- Diallyl sulfide from garlic suppresses quorum-sensing systems of Pseudomonas aeruginosa and enhances biosynthesis of three B vitamins through its thioether group.Microbial biotechnology · 2021Article
- Flavins Act as a Critical Liaison Between Metabolic Homeostasis and Oxidative Stress in the Retina.Frontiers in cell and developmental biology · 2020Review
- Gut Vibes in Parkinson's Disease: The Microbiota-Gut-Brain Axis.Movement disorders clinical practice · 2019Review
- Maternal diet deficient in riboflavin induces embryonic death associated with alterations in the hepatic proteome of duck embryos.Nutrition & metabolism · 2019Article
- Flavin homeostasis in the mouse retina during aging and degeneration.The Journal of nutritional biochemistry · 2018Article
- Overview on the Bacterial Iron-Riboflavin Metabolic Axis.Frontiers in microbiology · 2018Review
- Article
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Authors and funding
8 authors.
Funding
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Abstract
Riboflavin deficiency is widespread in many regions over the world, especially in underdeveloped countries. In this study, we investigated the effects of riboflavin deficiency on protein expression profiles in HepG2 cells in order to provide molecular information for the abnormalities induced by riboflavin deficiency. HepG2 cells were cultured in media containing different concentrations of riboflavin. Changes of cell viability and apoptosis were assessed. A comparative proteomic analysis was performed using a label-free shotgun method with LC-MS/MS to investigate the global changes of proteomic profiles in response to riboflavin deficiency. Immunoblotting test was used to validate the results of proteomic approach. The cell viability and apoptosis tests showed that riboflavin was vital in maintaining the cytoactivity of HepG2 cells. The label-free proteomic analysis revealed that a total of 37 proteins showing differential expression (±2 fold, p < 0.05) were identified after riboflavin deficiency. Bioinformatics analysis indicated that the riboflavin deficiency caused an up-regulation of Parkinson's disease pathway, steroid catabolism, endoplasmic reticulum stress and apoptotic process, while the fatty acid metabolism, tricarboxylic citrate cycle, oxidative phosphorylation and iron metabolism were down-regulated. These findings provide a molecular basis for the elucidation of the effects caused by riboflavin deficiency.
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