Evidence map›Paper›PMID 28367479›Full record

ArticleBio-protocol2017

Heterochronic Pellet Assay to Test Cell-cell Communication in the Mouse Retina.

Nobuhiko Tachibana, Dawn Zinyk, Randy Ringuette, Valerie Wallace, Carol Schuurmans

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nobuhiko TachibanaBiological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, Canada; Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, Canada.
Dawn ZinykBiological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, Canada; Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, Canada.
Randy RinguetteDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada.
Valerie WallaceDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, Canada; Department of Ophthalmology and Vision Sciences and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.
Carol SchuurmansBiological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, Canada; Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, Canada.

Funding

CIHR 142338-1
6 · The paper itself

Abstract

All seven retinal cell types that make up the mature retina are generated from a common, multipotent pool of retinal progenitor cells (RPCs) (Wallace, 2011). One way that RPCs know when sufficient numbers of particular cell-types have been generated is through negative feedback signals, which are emitted by differentiated cells and must reach threshold levels to block additional differentiation of that cell type. A key assay to assess whether negative feedback signals are emitted by differentiated cells is a heterochronic pellet assay in which early stage RPCs are dissociated and labeled with BrdU, then mixed with a 20-fold excess of dissociated differentiated cells. The combined cells are then re-aggregated and cultured as a pellet on a membrane for 7-10 days

Indexed as

Amacrine cellHeterochronic pellet assayHeterotypic cell interactionsNegative feedback signalingRe-aggregationRetinal differentiationRetinal progenitor cells

Identifiers

PMID28367479
PMCPMC5373849

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.