Pulsed Dilution Method for the Recovery of Aggregated Mouse TNF-α.

Merat Mahmoodi, Maryam Ghodsi, Malihe Moghadam, Mojtaba Sankian

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Article in Reports of biochemistry & molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Merat MahmoodiImmuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran.

Maryam GhodsiImmuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran.

Malihe MoghadamImmuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran.

Mojtaba SankianImmuno-Biochemistry lab, Immunology Research Center, Buali Research Institute , School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe expression of mouse tumor necrosis factor alpha (TNF-α) in

methodsReducing agent and guanidine hydrochloride were used to solubilize inclusion bodies formed after TNF-(α) expression. Then, the refolding procedure was performed by pulsed dilution of the denatured protein into a refolding buffer. The properly-folded protein was purified by metal affinity chromatography.

resultsSDS-PAGE showed a 19.9 kDa band related to the mature TNF-(α) protein. The protein was recognized by anti-mouse TNF-(α) on western blots. The final concentration of the purified recombinant TNF-(α) was 62.5 µg/mL.

conclusionsOur study demonstrates the efficiency of this method to produce a high yield of folded mature TNF- (α).

Indexed as

Escherichia coliGuanidine HydrochlorideInclusion BodiesMouse TNF-(α)

Identifiers

PMID28367471

PMCPMC5346277

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.