ArticleResearch in microbiology
SlpE is a calcium-dependent cytotoxic metalloprotease associated with clinical isolates of Serratia marcescens.
Article in Research in microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- Fitness factor genes conserved within the multi-species core genome of Gram-negative Enterobacterales species contribute to bacteremia pathogenesis.PLoS pathogens · 2024Article
- Increased Proteolytic Activity ofPolish journal of microbiology · 2024Article
- Bioaerosols in swine confinement buildings: A metaproteomic view.Environmental microbiology reports · 2023Article
- Identification of a cytotoxic factor from a non-pigmented entomopathogenic Serratia marcescens isolate toxic towards human carcinoma cell lines.Archives of microbiology · 2023Article
- IgaA Protein, GumB, Has a Global Impact on the Transcriptome and Surface Proteome of Serratia marcescens.Infection and immunity · 2022Article
- The Role of SprIR Quorum Sensing System in the Regulation ofMicroorganisms · 2021Article
- Clearance of Gram-Negative Bacterial Pathogens from the Ocular Surface by Predatory Bacteria.Antibiotics (Basel, Switzerland) · 2021Article
- Biologically active pigment and ShlA cytolysin of Serratia marcescens induce autophagy in a human ocular surface cell line.BMC ophthalmology · 2020Article
- Sulfur Assimilation Alters Flagellar Function and Modulates the Gene Expression Landscape of Serratia marcescens.mSystems · 2019Article
- Serralysin family metalloproteases protects Serratia marcescens from predation by the predatory bacteria Micavibrio aeruginosavorus.Scientific reports · 2018Article
- CpxR-Dependent Thermoregulation of Serratia marcescens PrtA Metalloprotease Expression and Its Contribution to Bacterial Biofilm Formation.Journal of bacteriology · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Serralysin-like proteases are found in a wide variety of bacteria. These metalloproteases are frequently implicated in virulence and are members of the widely conserved RTX-toxin family. We identified a serralysin-like protease in the genome of a clinical isolate of Serratia marcescens that is highly similar to the canonical serralysin protein, PrtS. This gene was named serralysin-like protease E, SlpE, and was found in the majority (67%) of tested clinical isolates, but was absent from most tested non-clinical isolates including the insect pathogen and reference S. marcescens strain Db11. Purified recombinant SlpE exhibited calcium-dependent protease activity similar to metalloproteases PrtS and SlpB. Induction of slpE in the low-protease-producing S. marcescens strain PIC3611 highly elevated extracellular protease activity, and extracellular secretion required the lipD type 1 secretion system gene. Transcription of slpE was highly reduced in an eepR transcription factor mutant. Mutation of the slpE gene in a highly proteolytic clinical isolate reduced its protease activity, and evidence suggests that SlpE confers cytotoxicity of S. marcescens to the A549 airway carcinoma cell line. Together, these data reveal SlpE to be an EepR-regulated cytotoxic metalloprotease associated with clinical isolates of an important opportunistic pathogen.
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