ArticleCell stem cell2017
H3K4 Methylation-Dependent Memory of Somatic Cell Identity Inhibits Reprogramming and Development of Nuclear Transfer Embryos.
Article in Cell stem cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed.
- 5-Hydroxymethylcytosine Dynamics Reveals Coordinated Reprogramming of Parental Genomes and X Chromosome Dosage Balance in Mouse SCNT Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Overexpression of the pioneer transcription factor Nr5a2 promotes the development of mouse somatic cell nuclear transfer embryos.PLoS biology · 2026Article
- Pre-marking chromatin with H3K4 methylation is required for accurate zygotic genome activation and development.Nature communications · 2025Article
- Reversibility, regulation, and the community of development: the legacy of Sir John B. Gurdon.Nature communications · 2025Review
- Lycopene enhances epigenetic reprogramming and zygotic genome activation in the porcine somatic cell nuclear transfer embryo.Scientific reports · 2025Article
- Setd2 overexpression rescues bivalent gene expression during SCNT-mediated ZGA.Protein & cell · 2025Article
- Coordinated action of multiple active histone modifications shapes the zygotic genome activation in teleost embryos.Nature communications · 2025Article
- Differentiation success of reprogrammed cells is heterogeneous in vivo and modulated by somatic cell identity memory.Stem cell reports · 2025Article
- Sperm derived H2AK119ub1 is required for embryonic development in Xenopus laevis.Nature communications · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Cell reprogramming: methods, mechanisms and applications.Cell regeneration (London, England) · 2025Review
- Targeted DamID detects cell-type-specific histone modifications in intact tissues or organisms.PLoS biology · 2025Article
- Crossing epigenetic frontiers: the intersection of novel histone modifications and diseases.Signal transduction and targeted therapy · 2024Review
- DNA methylation regulates RNA mCell proliferation · 2024Article
- Organ transformation by environmental disruption of protein integrity and epigenetic memory in Drosophila.PLoS biology · 2024Article
- Canalizing cell fate by transcriptional repression.Molecular systems biology · 2024Review
- Histone post-translational modification and the DNA damage response.Genes & diseases · 2023Review
- Methylation of histone H3 lysine 4 is required for maintenance of beta cell function in adult mice.Diabetologia · 2023Article
- An acetylation-mediated chromatin switch governs H3K4 methylation read-write capability.eLife · 2023Article
- Somatic Cell Nuclear Transfer in Pigs.Methods in molecular biology (Clifton, N.J.) · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Vertebrate eggs can induce the nuclear reprogramming of somatic cells to enable production of cloned animals. Nuclear reprogramming is relatively inefficient, and the development of the resultant embryos is frequently compromised, in part due to the inappropriate expression of genes previously active in the donor nucleus. Here, we identify H3K4 methylation as a major epigenetic roadblock that limits transcriptional reprogramming and efficient nuclear transfer (NT). Widespread expression of donor-cell-specific genes was observed in inappropriate cell types in NT embryos, limiting their developmental capacity. The expression of these genes in reprogrammed embryos arises from epigenetic memories of a previously active transcriptional state in donor cells that is characterized by high H3K4 methylation. Reducing H3K4 methylation had little effect on gene expression in donor cells, but it substantially improved transcriptional reprogramming and development of NT embryos. These results show that H3K4 methylation imposes a barrier to efficient nuclear reprogramming and suggest approaches for improving reprogramming strategies.
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