ArticleColloids and surfaces. B, Biointerfaces2017
Rapid separation of bacteria from blood - Chemical aspects.
Article in Colloids and surfaces. B, Biointerfaces, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Recent Advances in Bacterial Separation and Enrichment from Blood for the Diagnosis of Bloodstream Infections.Sensors (Basel, Switzerland) · 2026Review
- LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia.Scientific reports · 2026Article
- Isolation and identification of bacteria from blood within 12 h using standard laboratory equipment.Scientific reports · 2025Article
- Next-Gen Nano Biosensor Technologies to Monitor Carbapenem Resistance for Personalized Medicine.Indian journal of microbiology · 2025Article
- Advances in Host Depletion and Pathogen Enrichment Methods for Rapid Sequencing-Based Diagnosis of Bloodstream Infection.The Journal of molecular diagnostics : JMD · 2024Review
- Growth and Distribution of Bacteria in Contaminated Whole Blood and Derived Blood Components.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2024Article
- A Review of Carbapenem Resistance inMicroorganisms · 2023Review
- Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Article
- Fluid-Screen as a real time dielectrophoretic method for universal microbial capture.Scientific reports · 2021Article
- Multilabel hybridization probes for sequence-specific detection of sepsis-related drug resistance genes in plasmids.Talanta open · 2021Article
- 3× multiplexed detection of antibiotic resistant plasmids with single molecule sensitivity.Lab on a chip · 2020Article
- Analysis of Identification Method for Bacterial Species and Antibiotic Resistance Genes Using Optical Data From DNA Oligomers.Frontiers in microbiology · 2020Article
- A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood.Micromachines · 2018Article
- Sequence-specific sepsis-related DNA capture and fluorescent labeling in monoliths prepared by single-step photopolymerization in microfluidic devices.Journal of chromatography. A · 2018Article
- Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes.Journal of chromatography. A · 2017Article
- Rapid separation of very low concentrations of bacteria from blood.Journal of microbiological methods · 2017Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
To rapidly diagnose infectious organisms causing blood sepsis, bacteria must be rapidly separated from blood, a very difficult process considering that concentrations of bacteria are many orders of magnitude lower than concentrations of blood cells. We have successfully separated bacteria from red and white blood cells using a sedimentation process in which the separation is driven by differences in density and size. Seven mL of whole human blood spiked with bacteria is placed in a 12-cm hollow disk and spun at 3000rpm for 1min. The red and white cells sediment more than 30-fold faster than bacteria, leaving much of the bacteria in the plasma. When the disk is slowly decelerated, the plasma flows to a collection site and the red and white cells are trapped in the disk. Analysis of the recovered plasma shows that about 36% of the bacteria is recovered in the plasma. The plasma is not perfectly clear of red blood cells, but about 94% have been removed. This paper describes the effects of various chemical aspects of this process, including the influence of anticoagulant chemistry on the separation efficiency and the use of wetting agents and platelet aggregators that may influence the bacterial recovery. In a clinical scenario, the recovered bacteria can be subsequently analyzed to determine their species and resistance to various antibiotics.
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