Evidence map›Paper›PMID 28364304›Full record

ArticlePharmaceutical research2017

Inter-Subject Variability in OCT1 Activity in 27 Batches of Cryopreserved Human Hepatocytes and Association with OCT1 mRNA Expression and Genotype.

Sarinj Fattah, Abhijit Babaji Shinde, Maja Matic, Myriam Baes, Ron H N van Schaik, Karel Allegaert, Celine Parmentier, Lysiane Richert, Patrick Augustijns, Pieter Annaert

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In one paragraph

Article in Pharmaceutical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Drug metabolism and disposition: the biological fate of chemicals · 2021
    Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Sarinj FattahDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Campus Gasthuisberg O&N II Herestraat 49 Box 921, 3000, Leuven, Belgium.
Abhijit Babaji ShindeLaboratory of Cell Metabolism, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.
Maja MaticDepartment Clinical Chemistry, Erasmus University Medical Centre, Rotterdam, Netherlands.
Myriam BaesLaboratory of Cell Metabolism, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.
Ron H N van SchaikDepartment Clinical Chemistry, Erasmus University Medical Centre, Rotterdam, Netherlands.
Karel AllegaertIntensive Care and Department of Surgery, Erasmus MC-Sophia Children's Hospital, Rotterdam, The Netherlands.
Celine ParmentierKaLy-Cell, Plobsheim, France.
Lysiane RichertKaLy-Cell, Plobsheim, France.
Patrick AugustijnsDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Campus Gasthuisberg O&N II Herestraat 49 Box 921, 3000, Leuven, Belgium.
Pieter AnnaertDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Campus Gasthuisberg O&N II Herestraat 49 Box 921, 3000, Leuven, Belgium. pieter.annaert@kuleuven.be.ORCID 0000-0003-3525-7351
KU Leuven · BEErasmus MC - Sophia Children’s Hospital · NLErasmus University Rotterdam · NLUniversité de franche-comté · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeOCT1/3 (Organic Cation Transporter-1 and -3; SLC22A1/3) are transmembrane proteins localized at the basolateral membrane of hepatocytes. They mediate the uptake of cationic endogenous compounds and/or xenobiotics. The present study was set up to verify whether the previously observed variability in OCT activity in hepatocytes may be explained by inter-individual differences in OCT1/3 mRNA levels or OCT1 genotype.

methodsTwenty-seven batches of cryopreserved human hepatocytes (male and female, age 24-88 y) were characterized for OCT activity, normalized OCT1/3 mRNA expression, and OCT1 genetic mutation. ASP

resultsASP

conclusionmRNA levels and genotype of OCT only partially explain inter-individual variability in OCT-mediated transport. Our findings illustrate the necessity of in vitro transporter activity profiling for better understanding of inter-individual drug disposition behavior.

Indexed as

AdultAgedAged, 80 and overBiological TransportFemaleFluorescent DyesGene ExpressionGenotypeHepatocytesHumansMaleMiddle AgedOptical ImagingOrganic Cation Transporter 1Organic Cation Transporter 3Organic Cation Transport Proteins1-methylpyridiniumFluorescent DyesOrganic Cation Transporter 1Organic Cation Transporter 3Organic Cation Transport ProteinsPyridinium CompoundsRNA, Messengerdrug uptake transportersfluorescent probe substratehuman hepatocytesmRNA expression and genetic polymorphismOrganic Cation Transporter (OCT)

Identifiers

PMID28364304
OpenAlexW2600388594

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.