ArticleApplied and environmental microbiology2017
Simulated rRNA/DNA Ratios Show Potential To Misclassify Active Populations as Dormant.
Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 84 citations in OpenAlex.
- From dividing to dormant: embracing the full activity spectrum for environmental microorganisms.Microbiology and molecular biology reviews : MMBR · 2026Review
- Targeting of Oral Bacterial Taxa Linked to Disease by Flavonols.Nutrients · 2026Article
- Shared stream-lake patterns in diversity, rRNA-based activity and community assembly of bacteria and microeukaryotes under distinct hydrological regimes.FEMS microbiology ecology · 2026Article
- Activation dynamics and assembly of root zone soil bacterial communities in response to stress-associated phytohormones.Microbiology spectrum · 2025Article
- Seasonally and niche-differentiated diversity of active, dormant and dead microbes in coastal waters and surface sediments.Communications biology · 2025Article
- Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols.Scientific reports · 2025Article
- A comparison of 16S rRNA-gene and 16S rRNA-transcript derived microbial communities in bulk and rhizosphere soils.Frontiers in microbiology · 2025Article
- A hunting ground for predatory bacteria at the Zhenbei seamount in the South China Sea.ISME communications · 2025Article
- The active free-living bathypelagic microbiome is largely dominated by rare surface taxa.ISME communications · 2024Article
- RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities.Microbiome · 2023Article
- Tracking the upstream history of aquatic microbes in a boreal lake yields new insights on microbial community assembly.PNAS nexus · 2022Article
- Organelle 16S rRNA amplicon sequencing enables profiling of active gut microbiota in murine model.Applied microbiology and biotechnology · 2022Article
- Spatio-Temporal Variation ofFrontiers in microbiology · 2022Article
- Concatenated 16S rRNA sequence analysis improves bacterial taxonomy.F1000Research · 2022Article
- RNA-Based Analysis Reveals High Diversity of Plant-Associated Active Fungi in the Atmosphere.Frontiers in microbiology · 2021Article
- Effects of Ocean Acidification on Resident and Active Microbial Communities ofFrontiers in microbiology · 2021Article
- Cyanobacteria and Algae in Clouds and Rain in the Area of puy de Dôme, Central France.Applied and environmental microbiology · 2020Article
- Leveraging eDNA to expand the study of hybrid zones.Molecular ecology · 2020Article
- Dormancy dampens the microbial distance-decay relationship.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota.Nature communications · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of rRNA/DNA ratios derived from surveys of rRNA sequences in RNA and DNA extracts is an appealing but poorly validated approach to infer the activity status of environmental microbes. To improve the interpretation of rRNA/DNA ratios, we performed simulations to investigate the effects of community structure, rRNA amplification, and sampling depth on the accuracy of rRNA/DNA ratios in classifying bacterial populations as "active" or "dormant." Community structure was an insignificant factor. In contrast, the extent of rRNA amplification that occurs as cells transition from dormant to growing had a significant effect (
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.