ReviewNature methods2017
A guide to light-sheet fluorescence microscopy for multiscale imaging.
Review in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 291 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
291 citing papers in PubMed.
- Convergent methodologies in prosthetic joint infection research: integrating transdisciplinary approaches to understand and prevent biofilm-driven failure of orthopaedic prostheses.Journal of medical microbiology · 2026Review
- Space-time light-sheet microscopy.Nature communications · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- In Vivo Light-Sheet Imaging of Senescence Reporter Activity in a Transparent Killifish.Bio-protocol · 2026Article
- Microscopy Cell Segmentation: Review and Benchmarking of Task-Specific and Foundation Models.Journal of imaging · 2026Review
- Protocol for ultrafast immunolabeling and 3D imaging of whole organs and large tissues.STAR protocols · 2026Article
- Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.Journal of biomedical optics · 2026Article
- Multispectral extended depth-of-field fluorescence microscopy with co-designed meta-optics and neural reconstruction.Light, science & applications · 2026Article
- NucVerse3D: generalizable 3D nuclear instance segmentation across heterogeneous microscopy modalities.Scientific reports · 2026Article
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Dynamic One-Dimensional Beam Shaping via a Mechanically Tunable Bilayer Metasurface.Nanophotonics (Berlin, Germany) · 2026Article
- A Novel Central-Peripheral Interface: The Auditory Nerve Glial Transition Zone Exhibits Enhanced Age-Related Immune and Glial Cell Dysfunction.bioRxiv : the preprint server for biology · 2026Article
- Microscopy in mitochondrial research - a comprehensive review.Journal of cell science · 2026Review
- ClearScope: A Fully Integrated Light-Sheet Theta Microscope for Sub-Micron-Resolution Imaging Without Lateral Size Constraints.Journal of imaging · 2026Article
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Biofilm Analysis by Confocal Microscopy-Basics and Practical Aspects.Microscopy research and technique · 2026Review
- Intravital microscopy for nanomedicine: investigating nanoparticle-tissue interactions in the native state.RSC advances · 2026Review
- A modular tissue-clearing framework integrated with light-field microscopy enables rapid volumetric phenotyping of cardiac tissue.Research square · 2026Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
231 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as developmental and cell biology, anatomical science, biophysics and neuroscience. Although adoption among biologists has been steady, LSFM has not displaced more traditional imaging methods despite its often-superior performance. One reason for this is that the field has largely conformed to a do-it-yourself ethic, although the challenges of big image data cannot be overstated. With the most powerful implementations of LSFM available to only a few groups worldwide, the scope of this technique is unnecessarily limited. Here we elucidate the key developments and define a simple set of underlying principles governing LSFM. In doing so, we aim to clarify the decisions to be made for those who wish to develop and use bespoke light-sheet systems and to assist in identifying the best approaches to apply this powerful technique to myriad biological questions.
Indexed as
Identifiers
28362435What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.