ArticleJournal of visualized experiments : JoVE2017
The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 90 citations in OpenAlex.
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- The circadian clock gene period regulates the composition and daily bacterial load of the gut microbiome in Drosophila melanogaster.Scientific reports · 2025Article
- Taste sensitivity and consumption responses to sucrose and artificial sweeteners inJournal of undergraduate research (Gainesville, Fla.) · 2025Article
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- Mating reconciles fitness and fecundity by switching diet preference in flies.Nature communications · 2024Article
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- Gut tumors in flies alter the taste valence of an anti-tumorigenic bitter compound.Current biology : CB · 2024Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated.
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