ArticleJournal of visualized experiments : JoVE2017
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Gasotransmitters bridging tumor biology and immunity: from pathophysiological insights to therapeutic potential.Frontiers in immunology · 2026Review
- Probing Subcellular Iron Availability with Genetically Encoded Nitric Oxide Biosensors.Biosensors · 2022Article
- Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels.Redox biology · 2022Article
- Single Donor Infusion of S-Nitroso-Human-Serum-Albumin Attenuates Cardiac Isograft Fibrosis and Preserves Myocardial Micro-RNA-126-3p in a Murine Heterotopic Heart Transplant Model.Transplant international : official journal of the European Society for Organ Transplantation · 2022Article
- Complexities of the chemogenetic toolkit: Differential mDAAO activation by d-amino substrates and subcellular targeting.Free radical biology & medicine · 2021Article
- Towards genetically encoded sensors for nitric oxide bioimaging in planta.Plant physiology · 2021Article
- Article
- Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes.STAR protocols · 2020Article
- Endothelial lipase increases eNOS activating capacity of high-density lipoprotein.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Article
- L-type CaScience signaling · 2020Article
- Discordance between eNOS phosphorylation and activation revealed by multispectral imaging and chemogenetic methods.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genetic biosensors for imaging nitric oxide in single cells.Free radical biology & medicine · 2018Review
- Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells.Nitric oxide : biology and chemistry · 2017Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
Nitric Oxide (NO•) is a small radical, which mediates multiple important cellular functions in mammals, bacteria and plants. Despite the existence of a large number of methods for detecting NO• in vivo and in vitro, the real-time monitoring of NO• at the single-cell level is very challenging. The physiological or pathological effects of NO• are determined by the actual concentration and dwell time of this radical. Accordingly, methods that allow the single-cell detection of NO• are highly desirable. Recently, we expanded the pallet of NO• indicators by introducing single fluorescent protein-based genetically encoded nitric oxide (NO•) probes (geNOps) that directly respond to cellular NO• fluctuations and, hence, addresses this need. Here we demonstrate the usage of geNOps to assess intracellular NO• signals in response to two different chemical NO•-liberating molecules. Our results also confirm that freshly prepared 3-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-propanamine (NOC-7) has a much higher potential to evoke change in intracellular NO• levels as compared with the inorganic NO• donor sodium nitroprusside (SNP). Furthermore, dual-color live-cell imaging using the green geNOps (G-geNOp) and the chemical Ca
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