Evidence map›Paper›PMID 28362372›Full record

ArticleJournal of visualized experiments : JoVE2017

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials.

Keith C Heyde, Felicia Y Scott, Sung-Ho Paek, Ruihua Zhang, Warren C Ruder

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Living Material with Temperature-Dependent Light Absorption.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Keith C HeydeDepartment of Mechanical Engineering, Carnegie Mellon University; Engineering Science and Mechanics Program, Virginia Polytechnic Institute and State University.
Felicia Y ScottDepartment of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
Sung-Ho PaekDepartment of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
Ruihua ZhangDepartment of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
Warren C RuderDepartment of Biological Systems Engineering, Virginia Polytechnic Institute and State University; Department of Bioengineering, University of Pittsburgh; warrenr@pitt.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed an abiotic-biotic interface that allows engineered cells to control the material properties of a functionalized surface. This system is made by creating two modules: a synthetically engineered strain of E. coli cells and a functionalized material interface. Within this paper, we detail a protocol for genetically engineering selected behaviors within a strain of E. coli using molecular cloning strategies. Once developed, this strain produces elevated levels of biotin when exposed to a chemical inducer. Additionally, we detail protocols for creating two different functionalized surfaces, each of which is able to respond to cell-synthesized biotin. Taken together, we present a methodology for creating a linked, abiotic-biotic system that allows engineered cells to control material composition and assembly on nonliving substrates.

Indexed as

Cell EngineeringEscherichia coliSynthetic Biology

Identifiers

PMID28362372
PMCPMC5409335

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.