ArticleScientific reports2017
Comparative Subcellular Proteomics Analysis of Susceptible and Near-isogenic Resistant Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV infection.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms,Frontiers in microbiology · 2023Review
- Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution.Genomics, proteomics & bioinformatics · 2022Article
- Identification of Key Genes Involved in Resistance to Early Stage of BmNPV Infection in Silkworms.Viruses · 2022Article
- Integration of Transcriptomic and Proteomic Analyses Reveals New Insights into the Regulation of Immune Pathways in Midgut ofInsects · 2022Article
- A Review on Transcriptional Responses of Interactions between Insect Vectors and Plant Viruses.Cells · 2022Review
- Proteomic Identification ofInternational journal of molecular sciences · 2021Article
- A Novel Digestive Proteinase Lipase Member H-A inInsects · 2020Article
- Quantitative label-free proteomic analysis reveals differentially expressed proteins in the digestive juice of resistant versus susceptible silkworm strains and their predicted impacts on BmNPV infection.Journal of proteomics · 2020Article
- Identification and Functional Analysis of BmNPV-Interacting Proteins FromFrontiers in microbiology · 2020Article
- Article
- Study on the Role ofInternational journal of molecular sciences · 2019Article
- Article
- Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection.Journal of insect science (Online) · 2019Article
- Knockdown ofFrontiers in microbiology · 2019Article
- Molecular and biochemical responses in the midgut of the silkworm, Bombyx mori, infected with Nosema bombycis.Parasites & vectors · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanism of silkworm resistance to Bombyx mori nucleopolyhedrovirus (BmNPV) infection remains largely unclear. Accumulating evidence suggests that subcellular fractionation combined with proteomics is an ideal technique to analyse host antiviral mechanisms. To clarify the anti-BmNPV mechanism of the silkworm, the near-isogenic line BC9 (resistant strain) and the recurrent parent P50 (susceptible strain) were used in a comparative subcellular proteomics study. Two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS) was conducted on proteins extracted from the cytosol, mitochondria, and microsomes of BmNPV-infected and control larval midguts. A total of 87 proteins were successfully identified from the three subcellular fractions. These proteins were primarily involved in energy metabolism, protein metabolism, signalling pathways, disease, and transport. In particular, disease-relevant proteins were especially changed in microsomes. After infection with BmNPV, differentially expressed proteins (DEPs) primarily appeared in the cytosolic and microsomal fractions, which indicated that these two fractions might play a more important role in the response to BmNPV infection. After removing genetic background and individual immune stress response proteins, 16 proteins were identified as potentially involved in repressing BmNPV infection. Of these proteins, the differential expression patterns of 8 proteins according to reverse transcription quantitative PCR (RT-qPCR) analyses were consistent with the 2-DE results.
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