ArticleScientific reports2017
Atomic resolution structure of serine protease proteinase K at ambient temperature.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
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- Energy filtering enables macromolecular MicroED data at sub-atomic resolution.Nature communications · 2025Article
- Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.Communications chemistry · 2025Article
- Energy filtering enables macromolecular MicroED data at sub-atomic resolution.bioRxiv : the preprint server for biology · 2024Article
- Structural resolution of a small organic molecule by serial X-ray free-electron laser and electron crystallography.Nature chemistry · 2023Article
- A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.Nature communications · 2023Article
- Ab initio phasing macromolecular structures using electron-counted MicroED data.Nature methods · 2022Article
- Serial macromolecular crystallography at ALBA Synchrotron Light Source.Journal of synchrotron radiation · 2022Article
- Isoprenoid-chained lipid EROCOCScientific reports · 2020Article
- The susceptibility of disulfide bonds towards radiation damage may be explained by S⋯O interactions.IUCrJ · 2020Article
- High-resolution crystal structures of a "half sandwich"-type Ru(II) coordination compound bound to hen egg-white lysozyme and proteinase K.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.Journal of applied crystallography · 2020Article
- Viscosity-adjustable grease matrices for serial nanocrystallography.Scientific reports · 2020Article
- Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review.Molecules (Basel, Switzerland) · 2019Review
- Article
- Synthesis of silver nanoparticles byJournal, genetic engineering & biotechnology · 2018Article
- Structural biology of G protein-coupled receptors: new opportunities from XFELs and cryoEM.Current opinion in structural biology · 2018Review
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic resolution structures (beyond 1.20 Å) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes. Serial femtosecond crystallography (SFX) has attracted surging interest by providing a route to bypass such challenges. Yet the progress on atomic resolution analysis with SFX has been rather slow. In this report, we describe the 1.20 Å resolution structure of proteinase K using 13 keV photon energy. Hydrogen atoms, water molecules, and a number of alternative side-chain conformations have been resolved. The increase in the value of B-factor in SFX suggests that the residues and water molecules adjacent to active sites were flexible and exhibited dynamic motions at specific substrate-recognition sites.
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