ArticlePlant methods2017
A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Independent roles of Arp2/3 complex and RIC4 protein in the control of epidermal cell shape.Planta · 2026Article
- The fate and function of theFrontiers in plant science · 2025Article
- LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance.The Plant cell · 2021Article
- TheFrontiers in plant science · 2021Article
- Coefficient of variation as an image-intensity metric for cytoskeleton bundling.Scientific reports · 2020Article
- Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-inFrontiers in plant science · 2020Article
- Brassinosteroids Inhibit Autotropic Root Straightening by Modifying Filamentous-Actin Organization and Dynamics.Frontiers in plant science · 2020Article
- Actin depolymerization is able to increase plant resistance against pathogens via activation of salicylic acid signalling pathway.Scientific reports · 2019Article
- Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCytoskeleton can be observed in live plant cells in situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression and advanced microscopy methods such as spinning disc confocal microscopy (SDCM) or variable angle epifluorescence microscopy (VAEM). Existing methods for quantifying cytoskeletal dynamics are often either based on laborious manual structure tracking, or depend on costly commercial software. Current automated methods also do not readily allow separate measurements of structure lifetime, lateral mobility, and spatial anisotropy of these parameters.
resultsWe developed a new freeware-based, operational system-independent semi-manual technique for analyzing VAEM or SDCM data, QuACK (Quantitative Analysis of Cytoskeletal Kymograms), and validated it on data from
conclusionsOur new method for evaluating plant cytoskeletal dynamics has several advantages over existing techniques. It is intuitive, rapid compared to fully manual approaches, based on the free ImageJ software (including macros we provide here for download), and allows measurement of multiple parameters. Our approach was already used to document unexpected differences in actin mobility in transgenic
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