ArticleGenes & development2017
Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells.
Article in Genes & development, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Advances in Epigenomic Sequencing and Their Applications in Cancer Diagnostics.Precision chemistry · 2025Review
- Simultaneous single-cell analysis of 5mC and 5hmC with SIMPLE-seq.Nature biotechnology · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.Clinical epigenetics · 2023Review
- Interpreting non-coding disease-associated human variants using single-cell epigenomics.Nature reviews. Genetics · 2023Review
- The computational power of the human brain.Frontiers in cellular neuroscience · 2023Review
- Mammalian DNA methylome dynamics: mechanisms, functions and new frontiers.Development (Cambridge, England) · 2022Review
- Single-cell omics: A new direction for functional genetic research in human diseases and animal models.Frontiers in genetics · 2022Review
- A probabilistic framework for cellular lineage reconstruction using integrated single-cell 5-hydroxymethylcytosine and genomic DNA sequencing.Cell reports methods · 2021Article
- Joint profiling of histone modifications and transcriptome in single cells from mouse brain.Nature methods · 2021Article
- Asymmetric inheritance of epigenetic states in asymmetrically dividing stem cells.Current opinion in cell biology · 2020Review
- Epigenomic Remodeling in Huntington's Disease-Master or Servant?Epigenomes · 2020Review
- DNA Methylation-Mediated Modulation of Endocytosis as Potential Mechanism for Synaptic Function Regulation in Murine Inhibitory Cortical Interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Simultaneous quantification of protein-DNA interactions and transcriptomes in single cells with scDam&T-seq.Nature protocols · 2020Article
- A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity.Nature communications · 2020Article
- An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome.Nature structural & molecular biology · 2019Article
- Structural basis for preferential binding of human TCF4 to DNA containing 5-carboxylcytosine.Nucleic acids research · 2019Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Mapping chromatin modifications at the single cell level.Development (Cambridge, England) · 2019Review
- 5-Formylcytosine to cytosine conversion by C-C bond cleavage in vivo.Nature chemical biology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
To understand mammalian active DNA demethylation, various methods have been developed to map the genomic distribution of the demethylation intermediates 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC). However, the majority of these methods requires a large number of cells to begin with. In this study, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-seq (scMAB-seq), capable of profiling 5fC and 5caC at genome scale using ∼100 cells and single cells, respectively. liMAB-seq analysis of preimplantation embryos reveals the oxidation of 5mC to 5fC/5caC and the positive correlation between chromatin accessibility and processivity of ten-eleven translocation (TET) enzymes. scMAB-seq captures the cell-to-cell heterogeneity of 5fC and 5caC and reveals the strand-biased distribution of 5fC and 5caC. scMAB-seq also allows the simultaneous high-resolution mapping of sister chromatid exchange (SCE), facilitating the study of this type of genomic rearrangement. Therefore, our study not only establishes new methods for the genomic mapping of active DNA demethylation using limited numbers of cells or single cells but also demonstrates the utilities of the methods in different biological contexts.
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