Evidence map›Paper›PMID 28358851›Full record

ArticlePloS one2017

5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm.

Qing-Zhao Zhang, Ke-Qing Zhao, Yang Wu, Xian-Hui Li, Chen Yang, Li-Min Guo, Chun-Hong Liu, Di Qu, Chun-Quan Zheng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 44 citations in OpenAlex.

  1. Assessment of therapeutic response to photodynamic therapy with the Zn-Phthalocyanine RLP068/Cl versus topical Clindamycin in patients affected by Hidradenitis Suppurativa: a comparative clinical pilot study.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024
    Article
  2. Review
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  4. Biofilm Producing Methicillin-ResistantPathogens (Basel, Switzerland) · 2024
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  13. Nonconventional Therapeutics againstMicrobiology spectrum · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Qing-Zhao ZhangDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China.
Ke-Qing ZhaoDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China.
Yang WuKey Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Science and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai, China.
Xian-Hui LiDepartment of Otolaryngology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Chen YangDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China.
Li-Min GuoDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China.
Chun-Hong LiuDepartment of Clinical Laboratory, Eye and ENT Hospital, Fudan University, Shanghai, China.
Di QuKey Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Science and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai, China.
Chun-Quan ZhengDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China.
Eye & ENT Hospital of Fudan University · CNShanghai Medical College of Fudan University · CNRuian People's Hospital · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Staphylococcus aureus (S. aureus) is hard to be eradicated, not only due to the emergence of antibiotic resistant strains but also because of its ability to form biofilm. Antibiotics are the major approach to treating biofilm infections, but their effects are unsatisfactory. One of the potential alternative treatments for controlling biofilm infections is photodynamic therapy (PDT), which requires the administration of photosensitizer, followed by light activation. 5-aminolevulinic acid (ALA), a natural photosensitizer prodrug, presents favorable characteristics, such as easy penetration and rapid clearance. These advantages enable ALA-based PDT (ALA-PDT) to be well-tolerated by patients and it can be repeatedly applied without cumulative toxicity or serious side effects. ALA-PDT has been proven to be an effective treatment for multidrug resistant pathogens; however, the study of its effect on S. aureus biofilm is limited. Here, we established our PDT system based on the utilization of ALA and a light-emitting diode, and we tested the effect of ALA-PDT on S. aureus biofilm as well as the combined effect of ALA-PDT and antibiotics on S. aureus biofilm. Our results showed that ALA-PDT has a strong antibacterial effect on S. aureus biofilm, which was confirmed by the confocal laser scanning microscope. We also found that lethal photosensitization occurred predominantly in the upper layer of the biofilm, while the residual live bacteria were located in the lower layer of the biofilm. In addition, the improved bactericidal effect was observed in the combined treatment group but in a strain-dependent manner. Our results suggest that ALA-PDT is a potential alternative approach for future clinical use to treat S. aureus biofilm-associated infections, and some patients may benefit from the combined treatment of ALA-PDT and antibiotics, but drug sensitivity testing should be performed in advance.

Indexed as

Aminolevulinic AcidAnti-Bacterial AgentsBiofilmsHumansMicrobial Sensitivity TestsPhotochemotherapyStaphylococcal InfectionsStaphylococcus aureusAminolevulinic AcidAnti-Bacterial Agents

Identifiers

PMID28358851
PMCPMC5373590
OpenAlexW2600273598

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.