ArticleScientific reports2017
Serine protease SP105 activates prophenoloxidase in Asian corn borer melanization, and is regulated by serpin-3.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 28 citations in OpenAlex.
- Acetylation enables a dual-targeting fungal effector to inhibit insect immunity and development.PLoS pathogens · 2026Article
- Enhancing the Survival of Ichneumonid ParasitoidInsects · 2025Article
- Regulatory networks of mRNAs and miRNAs involved in the immune response of diamondback moth, Plutella xylostella to fungal infection.BMC genomics · 2025Article
- Article
- Identification and gene expression analysis of serine proteases and their homologs in the Asian corn borer Ostrinia furnacalis.Scientific reports · 2023Article
- A teratocyte-specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase-activating system of its host Plutella xylostella.Insect molecular biology · 2022Article
- Immune Defense Mechanism ofInsects · 2022Article
- KPI5 Is Involved in the Regulation of the Expression of Antibacterial Peptide Genes and Hemolymph Melanization in the Silkworm,Frontiers in immunology · 2022Article
- Serpin-4 Facilitates Baculovirus Infection by Inhibiting Melanization in Asian Corn Borer,Frontiers in immunology · 2022Article
- Article
- Identification of a Conserved Prophenoloxidase Activation Pathway in Cotton BollwormFrontiers in immunology · 2020Article
- Molecular characterization of immune responses of Helicoverpa armigera to infection with the mermithid nematode Ovomermis sinensis.BMC genomics · 2019Article
- Clip domain prophenoloxidase activating protease is required for Ostrinia furnacalis Guenée to defend against bacterial infection.Developmental and comparative immunology · 2018Article
- Activation of Aedes aegypti prophenoloxidase-3 and its role in the immune response against entomopathogenic fungi.Insect molecular biology · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melanization reaction, resulting from the activation of prophenoloxidase, is a vital immune response in insects for encapsulating and killing the invasive organisms. Prophenoloxidase needs to be proteolytically activated by its upstream prophenoloxidase-activating protease (PAP) in melanization. Identification and characterization of PAPs facilitates the understanding of the molecular mechanisms involved in insect immunity. We here cloned a full-length cDNA for a serine protease, named as SP105, from Asian corn borer, Ostrinia furnacalis (Guenée). The open reading frame of SP105 encodes 424-amino acid residue protein with a 19-residue signal peptide. Sequence comparison indicates that SP105 is most similar to Manduca sexta PAP3, a defined prophenoloxidase-activating protease. qRT-PCR analysis showed that SP105 mRNA levels increased significantly after a bacterial injection. Recombinant SP105 directly cleaved and activated Asian corn borer prophenoloxidase and therefore acted as the prophenoloxidase-activating protease. Additionally, SP105 formed SDS-stable complexes with a serine protease inhibitor, serpin-3, and its activity in activating prophenoloxidase was efficiently inhibited by serpin-3. Our work thus illustrated a prophenoloxidase-activating protease and revealed its regulation by serpin-3. The results would allow further advances in the understanding of the melanization in Asian corn borer and other insects.
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