Evidence map›Paper›PMID 28357642›Full record

ArticleClinical oral investigations2018

Genome-wide gene expression profiling of tongue squamous cell carcinoma by RNA-seq.

Hai Xia Zhang, Ou Sheng Liu, Chao Deng, Yan He, Ye Qian Feng, Jin An Ma, Chun Hong Hu, Zhan Gui Tang

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical oral investigations, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Association ofBioscience reports · 2019
    Trial
  2. Transcriptomic Profile of Canine Mammary Ductal Carcinoma.International journal of molecular sciences · 2023
    Article
  3. Article
  4. Systematically Deciphering the Pharmacological Mechanism of Fructus Aurantii via Network Pharmacology.Evidence-based complementary and alternative medicine : eCAM · 2021
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hai Xia ZhangDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China.
Ou Sheng LiuDepartment of Orthodontics, Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, Hunan Province, 410078, China.
Chao DengDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China.
Yan HeDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China.
Ye Qian FengDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China.
Jin An MaDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China.
Chun Hong HuDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, China. huchunh@medmail.com.cn.
Zhan Gui TangDepartment of Oral and Maxillofacial Surgery, Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, Hunan Province, 410078, China. tang_zhangui5@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTongue squamous cell carcinoma (TSCC) is significantly more malignant than other type of oral squamous cell carcinoma (OSCC). In this study, we aimed to identify specific global gene expression signatures of TSCC to investigate the more invasive behavior of the deeply infiltrating cancer.

methodsUsing RNA-seq technology, we detected gene expression of 20 TSCCs, 20 matched paratumor tissues, and 10 healthy normal mucosa tissues. Enrichment analysis of gene ontology (GO) and pathway was conducted using online tools DAVID for the dysregulated genes. Additionally, we performed the quantitative real-time RT-PCR (qRT-PCR) to validate the findings of RNA-Seq in 10 samples of TSCC, matched paratumor, and normal mucosa, respectively.

resultsWe detected 252 differentially expressed genes (DEGs) between TSCC and matched paratumor tissue, including 117 up-regulated and 135 down-regulated genes. For comparison between TSCC and normal mucosa, 234 DEGS were identified, consisting of 67 up-regulated and 167 down-regulated genes. For both two comparisons, GO categories of muscle contraction (GO: 0006936), epidermis development (GO: 0008544), epithelial cell differentiation (GO: 0030855), and keratinization (GO: 0031424) were commonly enriched. Altered gene expression affected some cancer-related pathways, such as tight junction. The qRT-PCR validation showed that gene expression patterns of FOLR1, NKX3-1, TFF3, PIGR, NEFL, MMP13, and HMGA2 were fully in concordance with RNA-Seq results.

conclusionFindings in this study demonstrated the genetic and molecular alterations associated with TSCC, providing new clues for understanding the molecular mechanisms of TSCC pathogenesis.

Indexed as

Gene Expression ProfilingSequence Analysis, RNACarcinoma, Squamous CellDown-RegulationGenome-Wide Association StudyHumansReal-Time Polymerase Chain ReactionTongue NeoplasmsUp-RegulationGene expressionqRT-PCRRNA-seqTongue squamous cell carcinoma

Identifiers

PMID28357642

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.