ArticleApplied microbiology and biotechnology2017
A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica.
Article in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 57 citations in OpenAlex.
- Microbial cell factories for gamma-linolenic acid biosynthesis: metabolic engineering, downstream processing, and future perspectives.Bioresources and bioprocessing · 2026Review
- Appraisal of biohydrogenating probiotic enzymes for the synthesis of conjugated linoleic acid isomers.Frontiers in microbiology · 2026Review
- Review
- Biosynthesis of conjugated linoleic acid: current status and future perspectives.Bioresources and bioprocessing · 2025Review
- Untargeted metabolomics ofHeliyon · 2025Article
- Conjugated linoleic acid metabolite impact in colorectal cancer: a potential microbiome-based precision nutrition approach.Nutrition reviews · 2025Review
- Conjugated Linoleic Acid in Cancer Therapy.Current drug delivery · 2025Review
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Article
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- EngineeringSynthetic and systems biotechnology · 2022Review
- Lipid Readjustment inBiomolecules · 2022Article
- Engineering precursor pools for increasing production of odd-chain fatty acids inMetabolic engineering communications · 2021Article
- Metabolic Engineering for Unusual Lipid Production inMicroorganisms · 2020Review
- Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.Journal of industrial microbiology & biotechnology · 2020Review
- Review
- Article
- The metabolism and genetic regulation of lipids in the oleaginous yeast Yarrowia lipolytica.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Review
- Synthetic biology tools for engineering Yarrowia lipolytica.Biotechnology advances · 2018Review
- New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.Microbial cell factories · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of β-oxidation (pox1-6∆), the inability to store lipids as triglycerides (dga1∆ dga2∆ are1∆ lro1∆), and the overexpression of the Y. lipolytica ∆12-desaturase gene (YlFAD2) under the control of the constitutive pTEF promoter. All strains received two copies of the pTEF-oPAI or pPOX-oPAI expression cassettes; PAI encodes linoleic acid isomerase in Propionibacterium acnes. The strains were cultured in neosynthesis or bioconversion medium in flasks or a bioreactor. The strain combining the three modifications mentioned above showed the best results: when it was grown in neosynthesis medium in a flask, CLAs represented 6.5% of total fatty acids and in bioconversion medium in a bioreactor, and CLA content reached 302 mg/L. In a previous study, a CLA degradation rate of 117 mg/L/h was observed in bioconversion medium. Here, by eliminating β-oxidation, we achieved a much lower rate of 1.8 mg/L/h.
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