ArticleNucleic acids research1988
The down-regulation of the chicken cytoplasmic beta actin during myogenic differentiation does not require the gene promoter but involves the 3' end of the gene.
Article in Nucleic acids research, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- T cell-expressed Ift88 is required for proper thymocyte differentiation in mice.Physiological reports · 2024Article
- Host cis-mediated extinction of a retrovirus permissive for expression in embryonal stem cells during differentiation.Journal of virology · 1998Article
- Transcription of the muscle regulatory gene Myf4 is regulated by serum components, peptide growth factors and signaling pathways involving G proteins.The Journal of cell biology · 1991Article
- Ribosomal protein L27 is identical in chick and rat.Nucleic acids research · 1991Article
- A highly conserved enhancer downstream of the human MLC1/3 locus is a target for multiple myogenic determination factors.Nucleic acids research · 1990Article
- Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.The EMBO journal · 1990Article
- Promoter upstream elements of the chicken cardiac myosin light-chain 2-A gene interact with trans-acting regulatory factors for muscle-specific transcription.Molecular and cellular biology · 1989Article
- A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.The EMBO journal · 1989Article
- 5' flanking and first intron sequences of the human beta-actin gene required for efficient promoter activity.Nucleic acids research · 1989Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken cytoplasmic beta actin gene is ubiquitously expressed in all cell types. In terminally differentiated muscle cells, however, the concentration of beta actin specific mRNA is down-regulated to scarcely detectable levels. To test for gene regions which are involved in the muscle specific reduction of beta actin specific mRNA, the isolated complete chicken beta actin gene or chimeric gene constructs containing parts of the gene were stably transfected into the myogenic mouse cell line C2C12 and their transcriptional activity was compared in proliferating myoblasts and postmitotic myotubes. A hybrid construct containing the beta actin promoter fused to the bacterial CAT gene showed high and constitutive expression during myocyte differentiation. In contrast, constructs containing the SV40 early promoter linked to the 3' end of the beta actin gene led to a marked reduction of beta actin transcripts in differentiated C2C12 myotubes. The stability of beta actin mRNA was analyzed in actinomycin D treated cells and found to be virtually unchanged in myotubes as compared to myoblasts. These results suggest that a sequence element located in the 3' end or 3' flanking region of the beta actin gene confers the myotube specific down-regulation that is not primarily due to destabilization of mRNA.
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