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Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media.
Article in mSphere. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 4 syntheses or guidelines pooled it, 49 citations in OpenAlex.
- Pooled it
- Metabolism and gene sequence variation in Turicella otitidis implies its adaptability and pathogenicity in extra-otic infection: a systematic review.BMC infectious diseases · 2023Pooled it
- Pooled it
- A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for Microbiology.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Guideline
- Type I interferon signaling in hematopoietic cells impairs neutrophil antibacterial function in the middle ear during viral co-infection.Cell reports. Medicine · 2026Article
- Molecular Characterization of the Middle Ear Microbiome in Pediatric Otitis Media with Effusion: Diagnostic and Clinical Implications.Journal of clinical medicine · 2026Review
- Pulmonary mucoidMicrobiology spectrum · 2026Article
- Analysis of pathogen distribution and risk factors for extended antibiotic course in children with microbiological-based protracted bacterial bronchitis in southwest China.Frontiers in pediatrics · 2026Article
- Recovery of microbial DNA by agar-containing solution from extremely low-biomass specimens including skin.Scientific reports · 2023Article
- Bacterial diversity of middle ear cholesteatoma by 16S rRNA gene sequencing in China.Functional & integrative genomics · 2023Article
- Realising respiratory microbiomic meta-analyses: time for a standardised framework.Microbiome · 2023Article
- Screen the unforeseen: Microbiome-profiling for detection of zoonotic pathogens in wild rats.Transboundary and emerging diseases · 2022Article
- Predominant Bacterial and Viral Otopathogens Identified Within the Respiratory Tract and Middle Ear of Urban Australian Children Experiencing Otitis Media Are Diversely Distributed.Frontiers in cellular and infection microbiology · 2022Article
- The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes.PloS one · 2022Article
- Identification of the Bacterial Pathogens in Children with Otitis Media: A Study in the Northwestern Portuguese District of Braga.Microorganisms · 2021Article
- Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing.Plants (Basel, Switzerland) · 2021Article
- Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model.NPJ biofilms and microbiomes · 2021Article
- Commensal bacteria in the upper respiratory tract regulate susceptibility to infection.Current opinion in immunology · 2020Review
- Niche- and Gender-Dependent Immune Reactions in Relation to the Microbiota Profile in Pediatric Patients with Otitis Media with Effusion.Infection and immunity · 2020Article
- Upper Respiratory Tract Microbiome and Otitis Media Intertalk: Lessons from the Literature.Journal of clinical medicine · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. Outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged 5 to 42 months (median age, 19 months). The bacteriome profiles of middle ear fluid samples were determined by a nested-PCR amplification of the 16S rRNA gene (V4 region), followed by mass sequencing. The profiling results were compared to the results of specific PCR assays targeting selected prevalent pathogens. Bacteriome profiling using nested amplification of low-volume samples was aided by a bioinformatic subtraction of signal contaminants from the recombinant polymerase, achieving a sensitivity slightly lower than that of specific PCR detection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.