ArticleCellular and molecular bioengineering2017
Methods for 20S Immunoproteasome and 20S Constitutive Proteasome Determination Based on SPRI Biosensors.
Article in Cellular and molecular bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- An Array SPRi Biosensor for the Determination of Follicle-Stimulating Hormone in Blood Plasma.Sensors (Basel, Switzerland) · 2023Article
- Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis.International journal of molecular sciences · 2023Article
- Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome.Sensors (Basel, Switzerland) · 2021Article
- Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for ControlledMolecular & cellular proteomics : MCP · 2019Article
- PA28α/β Promote Breast Cancer Cell Invasion and Metastasis via Down-Regulation of CDK15.Frontiers in oncology · 2019Article
- Review
- Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors.Clinical and experimental immunology · 2018Article
Corrections and comments
- Erratum issuedCorrection to: Methods for 20S Immunoproteasome and 20S Constitutive Proteasome Determination Based on SPRI Biosensors.2019 · Anna, Sankiewicz [corrected to Sankiewicz, Anna]; Agnieszka, Markowska [corrected to Markowska, Agnieszka]; Zenon, Lukaszewski [corrected to Lukaszewski, Zenon]; Beata, Puzan [corrected to Puzan, Beata]; Ewa, Gorodkiewicz [corrected to Gorodkiewicz, Ewa]
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 20S proteasome, released into the circulation, is a novel cancer biomarker. It exists in two forms: the constitutive proteasome (20Sc) and the immunoproteasome (20Si), which both have separate diagnostic significance. The aim of this work was to develop new methods for 20Si and 20Sc determination. Five alternative specific biosensors usable with the surface plasmon resonance imaging (SPRI) technique for 20Si determination have been developed. Specific 20Si entrapment on the biosensor surface from an analyzed solution was achieved by means of an immobilized specific 20Si receptor. Four of the biosensors contain newly synthesized specific 20Si receptors, while the fifth contains the inhibitor ONX 0914. A method for 20Sc determination using an SPRI biosensor containing PSI inhibitor has been developed. By the introduction of an inhibitor blocking 20Si, 20Sc is selectively determined. All of the methods developed for 20Si and 20Sc determination exhibit good selectivity and satisfactory precision, recoveries and dynamic response ranges. 20Si and 20Sc were determined in blood plasma samples from healthy donors and patients with acute leukemia. In the case of these patients 20Si was the major component, and its level was more than one order of magnitude higher than in the healthy donors.
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Registered trials
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