Evidence map›Paper›PMID 28356424›Full record

ArticleMolecular biology of the cell2017

Bridging length scales to measure polymer assembly.

Bryan Kaye, Tae Yeon Yoo, Peter J Foster, Che-Hang Yu, Daniel J Needleman

Abstract read
In one paragraph

Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bryan KayeJohn A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138 bryankaye1@gmail.com.
Tae Yeon YooJohn A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138.
Peter J FosterJohn A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138.
Che-Hang YuJohn A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138.
Daniel J NeedlemanJohn A. Paulson School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Time-resolvable quantitative measurements of polymer concentration are very useful to elucidate protein polymerization pathways. There are numerous techniques to measure polymer concentrations in purified protein solutions, but few are applicable in vivo. Here we develop a methodology combining microscopy and spectroscopy to overcome the limitations of both approaches for measuring polymer concentration in cells and cell extracts. This technique is based on quantifying the relationship between microscopy and spectroscopy measurements at many locations. We apply this methodology to measure microtubule assembly in tissue culture cells and

Indexed as

AnimalsFluorescence Resonance Energy TransferFluorescent DyesMicroscopy, FluorescenceMicrotubulesPhotonsPolymerizationPolymersSpectrometry, FluorescenceTubulinXenopus laevisFluorescent DyesPolymersTubulin

Identifiers

PMID28356424
PMCPMC5426851

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.