ReviewCirculation research2017
Analysis of Cardiac Myocyte Maturation Using CASAAV, a Platform for Rapid Dissection of Cardiac Myocyte Gene Function In Vivo.
Abstract readReview
In one paragraph
Review in Circulation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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0cells of the map it votes in
90citing papers in PubMed
6.0field-weighted citation impact, top 3% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
90 citing papers in PubMed, 139 citations in OpenAlex.
- Right ventricular pressure overload disturbs T-tubule maturation via MEF2D's transcriptional regulation of BIN1.JCI insight · 2026Article
- Spatially guided in vivo single-cell functional genomics of postnatal heart.Nature cardiovascular research · 2026Article
- MYOM1 deficiency leads to dilated cardiomyopathy by disrupting the homeostasis of sarcoplasmic reticulum.Redox biology · 2026Article
- Spastin-mediated severing of glutamylated microtubules controls cardiomyocyte coupling.Nature cardiovascular research · 2026Article
- Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System.Cell proliferation · 2026Article
- Gene Editing Applications as Future Cardiovascular Therapies.Annual review of genetics · 2025Review
- Junctophilin-2 Regulates Store-Operated Calcium Entry to Drive Cardiac Fibroblast Activation, Fibrotic Repair, and Angiogenesis After Myocardial Infarction.Circulation · 2025Article
- Functional analysis of JPH2-knockout cardiomyocytes identifies ECCD as a novel indicator in a human cardiac modelJPH2.Stem cell research & therapy · 2025Article
- Characterization of theFASEB bioAdvances · 2025Article
- Rapid and robust generation of cardiomyocyte-specific crispants in zebrafish using the cardiodeleter system.Cell reports methods · 2025Article
- Review
- Preventing Site-Specific Calpain Proteolysis of Junctophilin-2 Protects Against Stress-Induced Excitation-Contraction Uncoupling and Heart Failure Development.Circulation · 2025Article
- Cardiac Applications of CRISPR/AAV-Mediated Precise Genome Editing.bioRxiv : the preprint server for biology · 2024Article
- In vivo proximity proteomics uncovers palmdelphin (PALMD) as a Z-disc-associated mitigator of isoproterenol-induced cardiac injury.Acta pharmacologica Sinica · 2024Article
- Junctophilin-2 is a double-stranded RNA-binding protein that regulates cardiomyocyte-autonomous innate immune response.Biochemical and biophysical research communications · 2024Article
- Non-Cell-Autonomous Cardiomyocyte Regulation Complicates Gene Supplementation Therapy forJACC. Basic to translational science · 2024Article
- Review
- Epigenetic Regulation of Cardiomyocyte Maturation by Arginine Methyltransferase CARM1.Circulation · 2024Article
- Ptpn23 Controls Cardiac T-Tubule Patterning by Promoting the Assembly of Dystrophin-Glycoprotein Complex.Circulation · 2024Article
- Structure, Function, and Regulation of the Junctophilin Family.Annual review of physiology · 2024Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 3 countries.
Yuxuan GuoFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Nathan J VanDusenFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Lina ZhangFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Weiliang GuFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Isha SethiFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Silvia GuatimosimFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Qing MaFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Blake D JardinFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Yulan AiFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Donghui ZhangFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Biyi ChenFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Ang GuoFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Guo-Cheng YuanFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
Long-Sheng SongFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.).
William T PuFrom the Cardiology, Boston Children's Hospital, MA (Y.G., N.J.V., Q.M., B.D.J., Y.A., D.Z., W.T.P.); Institute of Basic Medicine (L.Z.) and Pharmacology, School of Pharmacy (W.G.), Shanghai University of Traditional Chinese Medicine, China; Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA (I.S., G.-C.Y.); Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (S.G.); Cardiovascular Medicine, Department of Internal Medicine, François M. Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City (B.C., A.G., L.-S.S.); Veterans Affairs Medical Center, Iowa City (L.-S.S.); and Harvard Stem Cell Institute, Cambridge, MA (W.T.P.). wpu@pulab.org.
Veterans Health Administration · USShanghai University of Traditional Chinese Medicine · CNDana-Farber Cancer Institute · USUniversidade Federal de Minas Gerais · BR
Funding
Administrative Coordinating Center: Cardiovascular Development and Pediatric Cardiac Genomics ConsortiaU01HL131003 · NHLBI · CINCINNATI CHILDRENS HOSP MED CTR · PI CNOTA, JAMES, OLLBERDING, NICHOLAS J · 2016 to 2024
$54.8MRESEARCH METHODS IN PEDIATRICS HEART DISEASET32HL007572 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI Sarah D DE FERRANTI, Jane W. Newburger · 1985 to 2026
$13.0MRegulation of Cardiac Development in Health and DiseaseUM1HL098166 · NHLBI · HARVARD MEDICAL SCHOOL · PI PENNACCHIO, LEN ALEXANDER, PU, WILLIAM TSWENCHING · 2015 to 2019
$4.4MNovel Functions of the E-C Coupling Structural Protein Junctophilin-2 in the HeartR01HL130346 · NHLBI · UNIVERSITY OF IOWA · PI SONG, LONG-SHENG · 2016 to 2023
$4.4MT-tubule remodeling and Ca2+-dependent arrhythmogenesis in cardiomyopathies.R01HL090905 · NHLBI · UNIVERSITY OF IOWA · PI SONG, LONG-SHENG · 2008 to 2017
$4.0MYAP1 Regulation of cardiomyocyte proliferation, function, and regenerationR01HL116461 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI PU, WILLIAM TSWENCHING · 2013 to 2017
$2.6MFunctional dissection of the regulatory network that governs cardiomyocyte maturation.K99HL143194 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI VANDUSEN, NATHAN JAMES · 2019 to 2021
$232kIdentification and analysis of factors that regulate cardiomyocyte maturationF32HL134235 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI VANDUSEN, NATHAN JAMES · 2016 to 2017
$73kNHLBI NIH HHS F32 HL134235NHLBI NIH HHS K99 HL143194NHLBI NIH HHS R01 HL090905NHLBI NIH HHS R01 HL116461NHLBI NIH HHS R01 HL130346NHLBI NIH HHS T32 HL007572NHLBI NIH HHS U01 HL131003NHLBI NIH HHS UM1 HL098166
Abstract
rationaleLoss-of-function studies in cardiac myocytes (CMs) are currently limited by the need for appropriate conditional knockout alleles. The factors that regulate CM maturation are poorly understood. Previous studies on CM maturation have been confounded by heart dysfunction caused by whole organ gene inactivation.
objectiveTo develop a new technical platform to rapidly characterize cell-autonomous gene function in postnatal murine CMs and apply it to identify genes that regulate transverse tubules (T-tubules), a hallmark of mature CMs. METHODS AND
resultsWe developed CRISPR/Cas9/AAV9-based somatic mutagenesis, a platform in which AAV9 delivers tandem guide RNAs targeting a gene of interest and cardiac troponin-T promoter-driven Cre to
conclusionsCRISPR/Cas9/AAV9-based somatic mutagenesis is a powerful tool to study cell-autonomous gene functions. Genetic mosaics are invaluable to accurately define cell-autonomous gene function. JPH2 has a minor role in normal T-tubule maturation but is required to stabilize T-tubules in the failing heart. RYR2 is a novel T-tubule maturation factor.
Indexed as
AnimalsCell Growth ProcessesCells, CulturedCRISPR-Cas SystemsDependovirusGene EditingMembrane ProteinsMiceMice, TransgenicMuscle ProteinsMyocytes, CardiacJP2 protein, mouseMembrane ProteinsMuscle Proteinsallelesgene silencingheart failurematurationmutagenesistransverse tubule
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