ArticleCell reports2017
Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Proteomic investigation of signaling dynamics: from static maps to network rewiring.Bioscience reports · 2026Review
- HNature chemical biology · 2026Article
- [A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells].Se pu = Chinese journal of chromatography · 2024Article
- GRB2 promotes thymocyte positive selection by facilitating THEMIS-mediated inactivation of SHP1.The Journal of experimental medicine · 2023Article
- Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery.Cell reports methods · 2023Review
- Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway.Frontiers in immunology · 2023Article
- Unveiling the complexity of transcription factor networks in hematopoietic stem cells: implications for cell therapy and hematological malignancies.Frontiers in oncology · 2023Review
- Proximity labeling and other novel mass spectrometric approaches for spatiotemporal protein dynamics.Expert review of proteomics · 2021Review
- Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics.Nature communications · 2021Article
- Approaching complexity: systems biology and ms-based techniques to address immune signaling.Expert review of proteomics · 2020Review
- ANPELA: analysis and performance assessment of the label-free quantification workflow for metaproteomic studies.Briefings in bioinformatics · 2020Article
- Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics.Nature immunology · 2019Article
- Simultaneous Improvement in the Precision, Accuracy, and Robustness of Label-free Proteome Quantification by Optimizing Data Manipulation Chains.Molecular & cellular proteomics : MCP · 2019Article
- Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS.Molecular & cellular proteomics : MCP · 2019Article
- CD5, an Undercover Regulator of TCR Signaling.Frontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
20 authors at 8 institutions in 4 countries.
Funding
Abstract
Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and affinity purification (AP)-SWATH mass spectrometry to profile the dynamics of 53 high-confidence protein interactions in primary T cells, using the scaffold protein GRB2 as a model. The workflow also provided a sufficient level of robustness to pinpoint differential interaction dynamics between two similar, but functionally distinct, primary T cell populations. Altogether, we demonstrated that precise and reproducible quantitative measurements of protein interaction dynamics can be achieved in primary cells isolated from mammalian tissues, allowing resolution of the tissue-specific context of cell-signaling events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.