ArticleBiophysical journal2017
Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed.
- Interplay of chromatin organization and mechanics of the cell nucleus.Biophysical journal · 2024Article
- Article
- Extreme transport of light in spheroids of tumor cells.Nature communications · 2023Article
- Differential contribution of nuclear size scaling mechanisms between Xenopus species.Development, growth & differentiation · 2022Article
- PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers.Materials advances · 2022Article
- Specificity of Nuclear Size Scaling in Frog Erythrocytes.Frontiers in cell and developmental biology · 2022Article
- DNA content contributes to nuclear size control inMolecular biology of the cell · 2020Article
- Isolated nuclei stiffen in response to low intensity vibration.Journal of biomechanics · 2020Article
- Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells.Scientific reports · 2020Article
- Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective.Frontiers in bioengineering and biotechnology · 2020Review
- Effect of Nuclear Stiffness on Cell Mechanics and Migration of Human Breast Cancer Cells.Frontiers in cell and developmental biology · 2020Article
- Recapitulation of molecular regulators of nuclear motion during cell migration.Cell adhesion & migration · 2019Review
- Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules.Micromachines · 2019Review
- Mechanical principles of nuclear shaping and positioning.The Journal of cell biology · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Understanding the physical mechanisms governing nuclear mechanics is important as it can impact gene expression and development. However, how cell nuclei respond to external cues such as heat is not well understood. Here, we studied the material properties of isolated nuclei in suspension using an optical stretcher. We demonstrate that isolated nuclei regulate their volume in a highly temperature-sensitive manner. At constant temperature, isolated nuclei behaved like passive, elastic and incompressible objects, whose volume depended on the pH and ionic conditions. When the temperature was increased suddenly by even a few degrees Kelvin, nuclei displayed a repeatable and reversible temperature-induced volume transition, whose sign depended on the valency of the solvent. Such phenomenon is not observed for nuclei subjected to slow heating. The transition temperature could be shifted by adiabatic changes of the ambient temperature, and the magnitude of temperature-induced volume transition could be modulated by modifying the chromatin compaction state and remodeling processes. Our findings reveal that the cell nucleus can be viewed as a highly charged polymer gel with intriguing thermoresponsive properties, which might play a role in nuclear volume regulation and thermosensing in living cells.
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Registered trials
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