ArticlePLoS genetics2017
Salmonella genomic island 1 (SGI1) reshapes the mating apparatus of IncC conjugative plasmids to promote self-propagation.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- Conjugative transfer inhibition of IncA and IncC plasmids by SGI1-like elements via relaxosome assembly interference.Nucleic acids research · 2026Article
- Genetic Elements Associated with the Acquired Resistome of the Gut Microbiota in a Broiler Rooster Flock in Hungary.Animals : an open access journal from MDPI · 2026Article
- Interactions and evolutionary relationships among bacterial mobile genetic elements.Nature reviews. Microbiology · 2025Review
- Surface exclusion of IncC conjugative plasmids and their relatives.PLoS genetics · 2024Article
- Hijackers, hitchhikers, or co-drivers? The mysteries of mobilizable genetic elements.PLoS biology · 2024Article
- Multidrug resistance inApplied and environmental microbiology · 2024Article
- Two birds with one stone: SGI1 can stabilize itself and expel the IncC helper by hijacking the plasmid parABS system.Nucleic acids research · 2024Article
- Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.Microbiology spectrum · 2024Article
- Genomic Diversity, Antimicrobial Resistance, Plasmidome, and Virulence Profiles ofAntibiotics (Basel, Switzerland) · 2023Article
- A systematic approach to classify and characterize genomic islands driven by conjugative mobility using protein signatures.Nucleic acids research · 2023Article
- Conjugative IncC Plasmid Entry Triggers the SOS Response and Promotes Effective Transfer of the Integrative Antibiotic Resistance Element SGI1.Microbiology spectrum · 2023Article
- Overview ofFrontiers in microbiology · 2022Article
- Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.Molecular microbiology · 2021Article
- Crucial role of Salmonella genomic island 1 master activator in the parasitism of IncC plasmids.Nucleic acids research · 2021Article
- Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids.PLoS genetics · 2021Article
- Specificity and Selective Advantage of an Exclusion System in the Integrative and Conjugative Element ICEJournal of bacteriology · 2021Article
- IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1.Nucleic acids research · 2021Article
- Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota.Frontiers in microbiology · 2021Review
- Antimicrobial resistance in enteric bacteria: current state and next-generation solutions.Gut microbes · 2020Review
- Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.Nature microbiology · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IncC conjugative plasmids and Salmonella genomic island 1 (SGI1) and relatives are frequently associated with multidrug resistance of clinical isolates of pathogenic Enterobacteriaceae. SGI1 is specifically mobilized in trans by IncA and IncC plasmids (commonly referred to as A/C plasmids) following its excision from the chromosome, an event triggered by the transcriptional activator complex AcaCD encoded by these helper plasmids. Although SGI1 is not self-transmissible, it carries three genes, traNS, traHS and traGS, coding for distant homologs of the predicted mating pore subunits TraNC, TraHC and TraGC, respectively, encoded by A/C plasmids. Here we investigated the regulation of traNS and traHGS and the role of these three genes in the transmissibility of SGI1. Transcriptional fusion of the promoter sequences of traNS and traHGS to the reporter gene lacZ confirmed that expression of these genes is inducible by AcaCD. Mating experiments using combinations of deletion mutants of SGI1 and the helper IncC plasmid pVCR94 revealed complex interactions between these two mobile genetic elements. Whereas traNC and traHGC are essential for IncC plasmid transfer, SGI1 could rescue null mutants of each individual gene revealing that TraNS, TraHS and TraGS are functional proteins. Complementation assays of individual traC and traS mutants showed that not only do TraNS/HS/GS replace TraNC/HC/GC in the mating pore encoded by IncC plasmids but also that traGS and traHS are both required for SGI1 optimal transfer. In fact, remodeling of the IncC-encoded mating pore by SGI1 was found to be essential to enhance transfer rate of SGI1 over the helper plasmid. Furthermore, traGS was found to be crucial to allow DNA transfer between cells bearing IncC helper plasmids, thereby suggesting that by remodeling the mating pore SGI1 disables an IncC-encoded entry exclusion mechanism. Hence traS genes facilitate the invasion by SGI1 of cell populations bearing IncC plasmids.
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