ArticleFrontiers in neuroscience2017
Embedding and Chemical Reactivation of Green Fluorescent Protein in the Whole Mouse Brain for Optical Micro-Imaging.
Article in Frontiers in neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Brain-wide atlas of outputs and inputs of mouse auditory cortex revealed by modified viral labeling strategies.Fundamental research · 2026Article
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- Connecting single-cell transcriptomes to projectomes in mouse visual cortex.bioRxiv : the preprint server for biology · 2023Article
- Low-temperature resin embedding of the whole brain for various precise structures dissection.iScience · 2023Article
- Dissection of the long-range projections of specific neurons at the synaptic level in the whole mouse brain.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fluorescent transgenic mouse models for whole-brain imaging in health and disease.Frontiers in molecular neuroscience · 2022Review
- Plastic embedding for precise imaging of large-scale biological tissues labeled with multiple fluorescent dyes and proteins.Biomedical optics express · 2021Article
- Article
- Cellular anatomy of the mouse primary motor cortex.Nature · 2021Article
- Divergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex.Neuroscience bulletin · 2021Article
- Cross-Streams Through the Ventral Posteromedial Thalamic Nucleus to Convey Vibrissal Information.Frontiers in neuroanatomy · 2021Article
- Anatomically revealed morphological patterns of pyramidal neurons in layer 5 of the motor cortex.Scientific reports · 2020Article
- RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.Nature methods · 2020Article
- Genetic Single Neuron Anatomy Reveals Fine Granularity of Cortical Axo-Axonic Cells.Cell reports · 2019Article
- Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex.Frontiers in neuroscience · 2019Article
- DeepBouton: Automated Identification of Single-Neuron Axonal Boutons at the Brain-Wide Scale.Frontiers in neuroinformatics · 2019Article
- Cell-type-specific and projection-specific brain-wide reconstruction of single neurons.Nature methods · 2018Article
- Penetration model for chemical reactivation for resin-embedded green fluorescent protein imaging.Journal of biomedical optics · 2018Article
- Simultaneous Acquisition of Multicolor Information From Neural Circuits in Resin-Embedded Samples.Frontiers in neuroscience · 2018Article
- Reconstruction of Intratelencephalic Neurons in the Mouse Secondary Motor Cortex Reveals the Diverse Projection Patterns of Single Neurons.Frontiers in neuroanatomy · 2018Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resin embedding has been widely applied to fixing biological tissues for sectioning and imaging, but has long been regarded as incompatible with green fluorescent protein (GFP) labeled sample because it reduces fluorescence. Recently, it has been reported that resin-embedded GFP-labeled brain tissue can be imaged with high resolution. In this protocol, we describe an optimized protocol for resin embedding and chemical reactivation of fluorescent protein labeled mouse brain, we have used mice as experiment model, but the protocol should be applied to other species. This method involves whole brain embedding and chemical reactivation of the fluorescent signal in resin-embedded tissue. The whole brain embedding process takes a total of 7 days. The duration of chemical reactivation is ~2 min for penetrating 4 μm below the surface in the resin-embedded brain. This protocol provides an efficient way to prepare fluorescent protein labeled sample for high-resolution optical imaging. This kind of sample was demonstrated to be imaged by various optical micro-imaging methods. Fine structures labeled with GFP across a whole brain can be detected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.