ArticleProteome science2016
Proteomic analysis of microparticles isolated from malaria positive blood samples.
Article in Proteome science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Pooled it
- Nuclear import of malaria RNA rewires splicing in host immune cells.Cell reports · 2026Article
- Review
- Guidelines for the purification and characterization of extracellular vesicles of parasites.Journal of extracellular biology · 2023Article
- Host-Derived Extracellular Vesicles in Blood and Tissue Human Protozoan Infections.Microorganisms · 2023Review
- Insights into Characteristic Volatiles in Wuyi Rock Teas with Different Cultivars by Chemometrics and Gas Chromatography Olfactometry/Mass Spectrometry.Foods (Basel, Switzerland) · 2022Article
- Extracellular Vesicles Derived from Early and Late StageJournal of clinical medicine · 2022Article
- Extracellular vesicles in malaria: an agglomeration of two decades of research.Malaria journal · 2021Review
- Multiparameter Flow Cytometry Analysis of the Human Spleen Applied to Studies of Plasma-Derived EVs FromFrontiers in cellular and infection microbiology · 2021Article
- Duration of Storage Reduced Erythrocytes Profiles andJournal of blood medicine · 2021Article
- The search for molecular mimicry in proteins carried by extracellular vesicles secreted by cells infected withCommunicative & integrative biology · 2021Article
- Review
- Extracellular Vesicles Could Carry an Evolutionary Footprint in Interkingdom Communication.Frontiers in cellular and infection microbiology · 2020Review
- Role of Extracellular Vesicles in Cellular Cross Talk in Malaria.Frontiers in immunology · 2020Review
- Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets.Journal of extracellular vesicles · 2020Article
- An Update on Isolation Methods for Proteomic Studies of Extracellular Vesicles in Biofluids.Molecules (Basel, Switzerland) · 2019Review
- Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation.PloS one · 2018Article
- Characterization ofFrontiers in microbiology · 2018Article
- Genome-wide identification of novel vaccine candidates for3 Biotech · 2017Article
- Proteomic analysis of extracellular vesicles from aWellcome open research · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma membrane derived extracellular vesicles (PMEVs) are subcellular structures that are generated when they bud off the plasma membrane. They can be found in healthy individuals but the numbers tend to increase in pathological conditions including malaria. Although, various studies have been carried out on the protein content of specific cellular derived MPs, there seems to be paucity of information on the protein content of circulating MPs in malaria and their association with the various signs and symptoms of the disease. The aim of this study was therefore to carry out proteomic analyses of MPs isolated from malaria positive samples and compare them with proteins of MPs from malaria parasite culture supernatant and healthy controls in order to ascertain the role of MPs in malaria infection.
methodsPlasma samples were obtained from forty-three (43) malaria diagnosed patients (cases) and ten (10) healthy individuals (controls). Malaria parasite culture supernatant was obtained from our laboratory and MPs were isolated from them and confirmed using flow cytometry. 2D LC-MS was done to obtain their protein content. Resultant data were analyzed using SPSS Ver. 21.0 statistical software, Kruskal Wallis test and Spearman's correlation coefficient
resultsIn all, 1806 proteins were isolated from the samples. The MPs from malaria positive samples recorded 1729 proteins, those from culture supernatant were 333 while the control samples recorded 234 proteins. The mean number of proteins in MPs of malaria positive samples was significantly higher than that in the control samples. Significantly, higher quantities of haemoglobin subunits were seen in MPs from malaria samples and culture supernatant compared to control samples.
conclusionA great number of proteins were observed to be carried in the microparticles (MPs) from malaria samples and culture supernatant compared to controls. The greater loss of haemoglobin from erythrocytes via MPs from malaria patients could serve as the initiation and progression of anaemia in
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