ArticlemBio2017
Comparison of Standard Culture-Based Method to Culture-Independent Method for Evaluation of Hygiene Effects on the Hand Microbiome.
Article in mBio, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- The Role of Gut Microbiota in Gastrointestinal Symptoms of Children with ASD.Medicina (Kaunas, Lithuania) · 2019Pooled it
- Longitudinal analysis of the hand microbiome in response to chlorine-based antiseptic use during a military field exercise.Microbiology spectrum · 2026Article
- Application of AI-facilitated handwashing monitoring system on food handlers.Scientific reports · 2026Article
- The Hygienic Significance of Microbiota and Probiotics for Human Wellbeing.Probiotics and antimicrobial proteins · 2025Review
- Review
- Effective use of skin microbiome signatures for fingerprint identification.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- The dynamic relationship between skin microbiomes and personal care products: A comprehensive review.Heliyon · 2024Review
- Daily occupational exposure in swine farm alters human skin microbiota and antibiotic resistome.iMeta · 2024Article
- A Novel Handrub Tablet Loaded with Pre- and Post-Biotic Solid Lipid Nanoparticles Combining Virucidal Activity and Maintenance of the Skin Barrier and Microbiome.Pharmaceutics · 2023Article
- Those Nematode-Trapping Fungi That are not Everywhere: Hints Towards Soil Microbial Biogeography.Journal of microbiology (Seoul, Korea) · 2023Article
- Genetic and Environmental Investigation of a Novel Phenylamino Acetamide Inhibitor of the Pseudomonas aeruginosa Type III Secretion System.Applied and environmental microbiology · 2023Article
- Evaluation of the Efficiency of Hand Hygiene Technique with Hydroalcoholic Solution by Image Color Summarize.Medicina (Kaunas, Lithuania) · 2022Article
- Viral infectious diseases severity: co-presence of transcriptionally active microbes (TAMs) can play an integral role for disease severity.Frontiers in immunology · 2022Review
- Combining Culture-Dependent and Culture-Independent Methods: New Methodology Insight on theFoods (Basel, Switzerland) · 2021Article
- Challenges in exploring and manipulating the human skin microbiome.Microbiome · 2021Review
- Free DNA and Metagenomics Analyses: Evaluation of Free DNA Inactivation Protocols for Shotgun Metagenomics Analysis of Human Biological Matrices.Frontiers in microbiology · 2021Article
- Young scientist perspective-microbiology trainees and social media: making science go viral during a pandemic.FEMS microbes · 2021Article
- Deployment of a Smart Handwashing Station in a School Setting During the COVID-19 Pandemic: Field Study.JMIR public health and surveillance · 2020Article
- The microbiota of the surface, dermis and subcutaneous tissue of dog skin.Animal microbiome · 2020Article
- Gut microbiome of endangered Tor putitora (Ham.) as a reservoir of antibiotic resistance genes and pathogens associated with fish health.BMC microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hands play a critical role in the transmission of microbiota on one's own body, between individuals, and on environmental surfaces. Effectively measuring the composition of the hand microbiome is important to hand hygiene science, which has implications for human health. Hand hygiene products are evaluated using standard culture-based methods, but standard test methods for culture-independent microbiome characterization are lacking. We sampled the hands of 50 participants using swab-based and glove-based methods prior to and following four hand hygiene treatments (using a nonantimicrobial hand wash, alcohol-based hand sanitizer [ABHS], a 70% ethanol solution, or tap water). We compared results among culture plate counts, 16S rRNA gene sequencing of DNA extracted directly from hands, and sequencing of DNA extracted from culture plates. Glove-based sampling yielded higher numbers of unique operational taxonomic units (OTUs) but had less diversity in bacterial community composition than swab-based sampling. We detected treatment-induced changes in diversity only by using swab-based samples (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.