ArticlePloS one2017
Sampling and detection of airborne influenza virus towards point-of-care applications.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Review
- Environmental metagenomics enhances detection of circulating viruses from live poultry markets in Cambodia.Nature communications · 2026Article
- Development of a Platform forAerosol science and technology : the journal of the American Association for Aerosol Research · 2026Article
- Research advances in microfluidic collection and detection of virus, bacterial, and fungal bioaerosols.Mikrochimica acta · 2024Review
- Capture and inactivation of viral particles from bioaerosols by electrostatic precipitation.iScience · 2023Article
- Article
- Novel Virus Air Sampler Based on Electrostatic Precipitation and Air Sampling of SARS-CoV-2.Microorganisms · 2023Article
- Article
- Biosensor detection of airborne respiratory viruses such as SARS-CoV-2.SLAS technology · 2022Review
- Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- Comparison of three air samplers for the collection of four nebulized respiratory viruses - Collection of respiratory viruses from air.Indoor air · 2021Article
- Recent advancements in the measurement of pathogenic airborne viruses.Journal of hazardous materials · 2021Review
- Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus.Scientific reports · 2021Article
- Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling.Journal of hazardous materials · 2021Article
- Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms.Micromachines · 2021Review
- Integration of sample preparation with RNA-Amplification in a hand-held device for airborne virus detection.Analytica chimica acta · 2021Article
- Facile Approaches of Polymeric Face Masks Reuse and Reinforcements for Micro-Aerosol Droplets and Viruses Filtration: A Review.Polymers · 2020Review
- Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling.Annals of work exposures and health · 2020Article
- COVID-19: impact on colorectal surgery.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland · 2020Article
- Bioaerosol Sampling: Classical Approaches, Advances, and Perspectives.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Airborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.