ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2017
3D visualization and quantification of microvessels in the whole ischemic mouse brain using solvent-based clearing and light sheet microscopy.
Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed, 129 citations in OpenAlex.
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- A spatiotemporal atlas of cerebrovascular development in zebrafish.Nature communications · 2026Article
- Tissue-type Differences in Focused Ultrasound and Microbubble-mediated Drug Delivery to the Brain Exist at Vessel Level.Theranostics · 2026Article
- PS$^{2}$2 F: Polarized Spiral Point Spread Function for Single-Shot 3D Sensing.IEEE transactions on pattern analysis and machine intelligence · 2025Article
- Arterial specification precedes microvascular restitution in the peri-infarct cortex that is driven by small microvessels.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2025Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- The Cerebrovascular Side of Plasticity: Microvascular Architecture across Health and Neurodegenerative and Vascular Diseases.Brain sciences · 2024Review
- A robust vessel-labeling pipeline with high tissue clearing compatibility for 3D mapping of vascular networks.iScience · 2024Article
- Deep learning-based vessel extraction in 3D confocal microscope images of cleared human glioma tissues.Biomedical optics express · 2024Article
- Longitudinal intravital imaging of mouse placenta.Science advances · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- The role of leptomeningeal collaterals in redistributing blood flow during stroke.PLoS computational biology · 2023Article
- Neural precursor cell delivery induces acute post-ischemic cerebroprotection, but fails to promote long-term stroke recovery in hyperlipidemic mice due to mechanisms that include pro-inflammatory responses associated with brain hemorrhages.Journal of neuroinflammation · 2023Article
- Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy.Anatomical science international · 2023Article
- Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic Lung.American journal of respiratory cell and molecular biology · 2023Article
- Transparent tissue in solid state for solvent-free and antifade 3D imaging.Nature communications · 2023Article
- Intrinsic, widefield optical imaging of hemodynamics in rodent models of Alzheimer's disease and neurological injury.Neurophotonics · 2023Review
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The visualization of cerebral microvessels is essential for understanding brain remodeling after stroke. Injection of dyes allows for the evaluation of perfused vessels, but has limitations related either to incomplete microvascular filling or leakage. In conventional histochemistry, the analysis of microvessels is limited to 2D structures, with apparent limitations regarding the interpretation of vascular circuits. Herein, we developed a straight-forward technique to visualize microvessels in the whole ischemic mouse brain, combining the injection of a fluorescent-labeled low viscosity hydrogel conjugate with 3D solvent clearing followed by automated light sheet microscopy. We performed transient middle cerebral artery occlusion in C57Bl/6j mice and acquired detailed 3D vasculature images from whole brains. Subsequent image processing, rendering and fitting of blood vessels to a filament model was employed to calculate vessel length density, resulting in 0.922 ± 0.176 m/mm
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.